Macroporous hydroxyapatite scaffolds for bone tissue engineering applications: Physicochemical characterization and assessment of rat bone marrow stromal cell viability

被引：58

作者：

Oliveira, Joaquim M. ^{[1
,2
,3
]}

Silva, Simone S. ^{[1
,2
]}

Malafaya, Patricia B. ^{[1
,2
]}

Rodrigues, Marcia T. ^{[1
,2
]}

Kotobuki, Noriko ^{[3
]}

Hirose, Motohiro ^{[3
]}

Gomes, Manuela E. ^{[1
,2
]}

Mano, Joao F. ^{[1
,2
]}

Ohgushi, Hajime ^{[3
]}

Reis, Rui L. ^{[1
,2
]}

机构：

[1] Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, Dept Polymer Engn, P-4710057 Braga, Portugal

[2] Inst Biotechnol & Bioengn, PT Govt Associated Lab, Braga, Portugal

[3] Natl Inst Adv Ind Sci & Technol, RICE, Amagasaki, Hyogo 6610974, Japan

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2009年 / 91A卷 / 01期

关键词：

hydroxyapatite; bone tissue engineering; rat bone marrow stromal; cells; scaffold; cell viability; IN-VIVO EVALUATION; MESENCHYMAL STEM-CELLS; OSTEOGENIC DIFFERENTIATION; IMPLANTS; FABRICATION; ADHESION; DEFECTS; DESIGN; VITRO; TRANSPLANTATION;

D O I：

10.1002/jbm.a.32213

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

In this work, a new methodology is reported for developing hydroxyapatite (HA) scaffolds using an organic sacrifice template. The novelty of work consists of possibility of obtaining porous and highly interconnected scaffolds mimicking the sacrificial component. Our purpose consisted of evaluating the physicochemical properties of the HA scaffolds by means of Fourier transform infra-red spectroscopy, X-ray diffraction analysis, and scanning electron microscopy (SEM) attached with an X-ray detector. The HA scaffolds obtained possess a porosity of similar to 70%, and macropores diameter in the range of 50-600 mu m. In contrast, results regarding the microcomputed tomography analysis have demonstrated both high pore uniformity and interconnectivity across the scaffolds. The compressive strength of the HA scaffolds was found to be 30.2 +/- 6.0 MPa. Bioactivity of the HA scaffolds was assessed by immersion into a simulated body fluid solution, in vitro. SEM observations have showed a deposition of apatite on the surface of the HA scaffolds, with a "cauliflower-like" morphology after 1 day, and tend to be more pronounced with the immersion time. The changes in calcium and phosphorus concentration were monitored by inductively-coupled plasma optical emission spectrometry. Cytotoxicity of the HA scaffolds was preliminarily investigated by carrying direct observation of mouse fibroblasts cells (L929 cell-line) death in the inverted microscope, and then cell viability was determined by means of carrying out a MTS assay. Complementarily, a luminescent cell viability assay based on the quantification of adenosine triphosphate was performed using rat bone marrow stromal cells (RBMSCs). A LIVE/DEAD assay and SEM analysis allowed the visualization of the RBMSCs adhesion and proliferation on the surface of the HA scaffolds. According to the results obtained from 3D architecture, mechanical properties, biocompatibility, and adhesion tests, it is suggested that HA scaffolds has potential to find applications in bone tissue engineering scaffolding. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 175-186, 2009

引用

页码：175 / 186

页数：12

共 47 条

[21]

Synthesis of Si, Mg substituted hydroxyapatites and their sintering behaviors [J].