Identification of phenotypic discriminating markers for intervertebral disc cells and articular chondrocytes

被引：74

作者：

Clouet, Johann ^{[1
]}

Grimandi, Gael ^{[1
]}

Pot-Vaucel, Marianne ^{[1
]}

Masson, Martial ^{[1
]}

Fellah, Hakim B. ^{[1
]}

Guigand, Lydie ^{[2
]}

Cherel, Yan ^{[2
]}

Bord, Eric ^{[3
]}

Rannou, Francois ^{[4
]}

Weiss, Pierre ^{[1
]}

Guicheux, Jerome ^{[1
]}

Vinatier, Claire ^{[1
,5
]}

机构：

[1] Univ Nantes, INSERM U791, Osteoarticular & Dent Tissue Engn Grp, F-44042 Nantes 1, France

[2] Univ Hosp, INRA UMR703, Natl Vet Sch Dev & Pathol Tissu Musculaire, Nantes, France

[3] Univ Hosp, Dept Neurosurg, Nantes, France

[4] Univ Paris 05, INSERM, U747, Paris, France

[5] GRAFTYS SAS, Aix En Provence, France

来源：

RHEUMATOLOGY | 2009年 / 48卷 / 11期

关键词：

Intervertebral disc; Nucleus pulposus; Annulus fibrosus; Chondrocyte; Phenotype; NUCLEUS PULPOSUS CELLS; CARTILAGE CELLS; DEGENERATION; EXPRESSION; MINERALIZATION; BIOCHEMISTRY; FIBROSUS; ARTERIES;

D O I：

10.1093/rheumatology/kep262

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Objective. The present study was conducted to improve our knowledge of intervertebral disc (IVD) cell biology by comparing the phenotype of nucleus pulposus (NP) and annulus fibrosus (AF) cells with that of articular chondrocytes (ACs). Methods. Rabbit cells from NP and AF were isolated and their phenotype was compared with that of AC by real-time PCR analysis of type I (COL1A1), II (COL2A1) and V (COL5A1) collagens, aggrecan transcript (AGC1), matrix Gla protein (MGP) and Htra serine peptidase 1 (Htra1). Results. Transcript analysis indicated that despite certain similarities, IVD cells exhibit distinct COL2A1/COL1A1 and COL2A1/AGC1 ratios as compared with AC. The expression pattern of COL5A1, MGP and Htra1 makes it possible to define a phenotypic signature for NP and AF cells. Conclusions. Our study shows that NP and AF cells exhibit a clearly distinguishable phenotype from that of AC. Type V collagen, MGP and HtrA1 could greatly help to discriminate among NP, AF and AC cells.

引用

页码：1447 / 1450

页数：4

共 26 条

[1]

What is intervertebral disc degeneration, and what causes it? [J].

Adams, Michael A. ;

Roughley, Peter J. .

SPINE, 2006, 31 (18) :2151-2161

[2]

Notochordal cells interact with nucleus pulposus cells: Regulation of proteoglycan synthesis [J].

Aguiar, DJ ;

Johnson, SL ;

Oegema, TR .

EXPERIMENTAL CELL RESEARCH, 1999, 246 (01) :129-137

[3]

Introduction - Disc degeneration: Summary [J].

An, HS ;

Anderson, PA ;

Haughton, VM ;

Iatridis, JC ;

Kang, JD ;

Lotz, JC ;

Natarajan, RN ;

Oegema, TR ;

Roughley, P ;

Setton, LA ;

Urban, JP ;

Videman, T ;

Andersson, GBJ ;

Weinstein, JN .

SPINE, 2004, 29 (23) :2677-2678

[4]

Anderson D Greg, 2005, Spine J, V5, p297S, DOI 10.1016/j.spinee.2005.02.019

[5]

Comparative gene expression profiling of normal and degenerative discs - Analysis of a rabbit annular laceration model [J].

Anderson, DG ;

Izzo, MW ;

Hall, DJ ;

Vaccaro, AR ;

Hilibrand, A ;

Arnold, W ;

Tuan, RS ;

Albert, TJ .

SPINE, 2002, 27 (12) :1291-1296

[6]

ELASTIC FIBERS IN HUMAN INTERVERTEBRAL DISKS [J].

BUCKWALTER, JA ;

COOPER, RR ;

MAYNARD, JA .

JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1976, 58 (01) :73-76

[7]

HtrA1: a novel regulator of physiological and pathological matrix mineralization? [J].

Canfield, A. E. ;

Hadfield, K. D. ;

Rock, C. F. ;

Wylie, E. C. ;

Wilkinson, F. L. .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2007, 35 :669-671

[8]

Eyre D R, 1979, Int Rev Connect Tissue Res, V8, P227

[9]

TYPE-1 AND TYPE-2 COLLAGENS IN INTERVERTEBRAL-DISK - INTERCHANGING RADIAL DISTRIBUTIONS IN ANNULUS FIBROSUS [J].

EYRE, DR ;

MUIR, H .

BIOCHEMICAL JOURNAL, 1976, 157 (01) :267-270

[10]

CD24 is expressed specifically in the nucleus pulposus of intervertebral discs [J].

Fujita, N ;

Miyamoto, T ;

Imai, JI ;

Hosogane, N ;

Suzuki, T ;

Yagi, M ;

Morita, K ;

Ninomiya, K ;

Miyamoto, K ;

Takaishi, H ;

Matsumoto, M ;

Morioka, H ;

Yabe, H ;

Chiba, K ;

Watanabe, S ;

Toyama, Y ;

Suda, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 338 (04) :1890-1896

← 1 2 3 →