Trehalose synthesis and metabolism are required at different stages of plant infection by Magnaporthe grisea

被引:195
作者
Foster, AJ [1 ]
Jenkinson, JM [1 ]
Talbot, NJ [1 ]
机构
[1] Univ Exeter, Washington Singer Labs, Sch Biol Sci, Exeter EX4 4QG, Devon, England
关键词
Magnaporthe grisea; metabolism; trehalase; virulence;
D O I
10.1093/emboj/cdg018
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The relationship of trehalose metabolism to fungal virulence was explored in the rice blast fungus Magnaporthe grisea. To determine the role of trehalose synthesis in pathogenesis, we identified and deleted TPS1, encoding trehalose-6-phosphate synthase. A Deltatps1 mutant failed to synthesize trehalose, sporulated poorly and was greatly attenuated in pathogenicity. Appressoria produced by Deltatps1 did not develop full turgor or elaborate penetration hyphae efficiently. To determine the role of subsequent trehalose breakdown, we deleted NTH1, which encodes a neutral trehalase. Nth1 mutants infected plants normally, but showed attenuated pathogenicity due to a decreased ability to colonize plant tissue. A second trehalase was also identified, required both for growth on trehalose and mobilization of intracellular trehalose during infection-related development. TRE1 encodes a cell wall-localized enzyme with characteristics of both neutral and acidic trehalases, but is dispensable for pathogenicity. Our results indicate that trehalose synthesis, but not its subsequent breakdown, is required for primary plant infection by M.grisea, while trehalose degradation is important for efficient development of the fungus in plant tissue following initial infection.
引用
收藏
页码:225 / 235
页数:11
相关论文
共 42 条
[11]  
DEVIRGILIO C, 1994, EUR J BIOCHEM, V219, P179
[12]  
DEVIRGILIO C, 1994, EUR J BIOCHEM, V219, P1
[13]   Independent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection by Magnaporthe grisea [J].
Dixon, KP ;
Xu, JR ;
Smirnoff, N ;
Talbot, NJ .
PLANT CELL, 1999, 11 (10) :2045-2058
[14]  
Felsenstein J., 1989, CLADISTICS, V5, P164, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X
[15]   Trehalose is required for the acquisition of tolerance to a variety of stresses in the filamentous fungus Aspergillus nidulans [J].
Fillinger, S ;
Chaveroche, MK ;
van Dijck, P ;
de Vries, R ;
Ruijter, G ;
Thevelein, J ;
d'Enfert, C .
MICROBIOLOGY-SGM, 2001, 147 :1851-1862
[16]   Gene discovery and gene function assignment in filamentous fungi [J].
Hamer, L ;
Adachi, K ;
Montenegro-Chamorro, MV ;
Tanzer, MM ;
Mahanty, SK ;
Lo, C ;
Tarpey, RW ;
Skalchunes, AR ;
Heiniger, RW ;
Frank, SA ;
Darveaux, BA ;
Lampe, DJ ;
Slater, TM ;
Ramamurthy, L ;
DeZwaan, TM ;
Nelson, GH ;
Shuster, JR ;
Woessner, J ;
Hamer, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (09) :5110-5115
[17]   THE ROLE OF TREHALOSE SYNTHESIS FOR THE ACQUISITION OF THERMOTOLERANCE IN YEAST .2. PHYSIOLOGICAL CONCENTRATIONS OF TREHALOSE INCREASE THE THERMAL-STABILITY OF PROTEINS IN-VITRO [J].
HOTTIGER, T ;
DEVIRGILIO, C ;
HALL, MN ;
BOLLER, T ;
WIEMKEN, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 219 (1-2) :187-193
[18]   PENETRATION OF HARD SUBSTRATES BY A FUNGUS EMPLOYING ENORMOUS TURGOR PRESSURES [J].
HOWARD, RJ ;
FERRARI, MA ;
ROACH, DH ;
MONEY, NP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11281-11284
[19]   Complementation of the Mpg1 mutant phenotype in Magnaporthe grisea reveals functional relationships between fungal hydrophobins [J].
Kershaw, MJ ;
Wakley, G ;
Talbot, NJ .
EMBO JOURNAL, 1998, 17 (14) :3838-3849
[20]  
KOPP M, 1993, J BIOL CHEM, V268, P4766