Cloning and characterization of a seed-specific ω-3 fatty acid desaturase cDNA from Perilla frutescens

被引:27
作者
Chung, CH [1 ]
Kim, JL [1 ]
Lee, YC [1 ]
Choi, YL [1 ]
机构
[1] Dong A Univ, Fac Life Sci & Resources, Div Biotechnol, Pusan 604714, South Korea
关键词
omega-3; desaturase; alpha-linolenic acid; perilla seeds; seed specificity;
D O I
10.1093/oxfordjournals.pcp.a029468
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A cDNA encoding omega-3 fatty acid desaturase was isolated from developing perilla seeds and characterized. On the basis of its deduced amino acid sequence comparison, this cDNA was assumed to be a new isoform of microsomal w-3 fatty acid desaturase gene. Accumulation of the mRNA for this cDNA showed seed-specific expression.
引用
收藏
页码:114 / 118
页数:5
相关论文
共 34 条
[1]   MAP-BASED CLONING OF A GENE CONTROLLING OMEGA-3-FATTY-ACID DESATURATION IN ARABIDOPSIS [J].
ARONDEL, V ;
LEMIEUX, B ;
HWANG, I ;
GIBSON, S ;
GOODMAN, HM ;
SOMERVILLE, CR .
SCIENCE, 1992, 258 (5086) :1353-1355
[2]   Two maize genes encoding ω-3 fatty acid desaturase and their differential expression to temperature [J].
Berberich, T ;
Harada, M ;
Sugawara, K ;
Kodama, H ;
Iba, K ;
Kusano, T .
PLANT MOLECULAR BIOLOGY, 1998, 36 (02) :297-306
[3]   NUCLEOTIDE-SEQUENCE OF A CDNA FROM LIMNANTHES-DOUGLASII L ENCODING A DELTA-15 LINOLEIC-ACID DESATURASE [J].
BHELLA, RS ;
MACKENZIE, SL .
PLANT PHYSIOLOGY, 1995, 108 (02) :861-861
[4]  
BROWSE J, 1993, J BIOL CHEM, V268, P16345
[5]  
Chung CH, 1996, MOL CELLS, V6, P108
[6]  
CHUNG CH, 1998, PLANT TISSUE CULT BI, V4, P42
[7]   CLONING OF A TEMPERATURE-REGULATED GENE ENCODING A CHLOROPLAST OMEGA-3 DESATURASE FROM ARABIDOPSIS-THALIANA [J].
GIBSON, S ;
ARONDEL, V ;
IBA, K ;
SOMERVILLE, C .
PLANT PHYSIOLOGY, 1994, 106 (04) :1615-1621
[8]   CLONING OF A CDNA-ENCODING TOBACCO OMEGA-3-FATTY-ACID DESATURASE [J].
HAMADA, T ;
KODAMA, K ;
NISHIMURA, M ;
IBA, K .
GENE, 1994, 147 (02) :293-294
[9]   cDNA cloning of a wounding-inducible gene encoding a plastid omega-3 fatty acid desaturase from tobacco [J].
Hamada, T ;
Nishiuchi, T ;
Kodama, H ;
Nishimura, M ;
Iba, K .
PLANT AND CELL PHYSIOLOGY, 1996, 37 (05) :606-611
[10]   Developmental regulation of genes for microsome and plastid ω-3 fatty acid desaturases in wheat (Triticum aestivum L.) [J].
Horiguchi, G ;
Kawakami, N ;
Kusumi, K ;
Kodama, H ;
Iba, K .
PLANT AND CELL PHYSIOLOGY, 1998, 39 (05) :540-544