Expression and assay of HECT domain ligases

被引：12

作者：

Beaudenon, S ^{[1
]}

Dastur, A

Huibregtse, JM

机构：

[1] Amb Diagnost, Austin, TX 78744 USA

[2] Univ Texas, Inst Cellular & Mol Biol, Austin, TX 78712 USA

来源：

UBIQUITIN AND PROTEIN DEGRADATION, PART A | 2005年 / 398卷

关键词：

D O I：

10.1016/S0076-6879(05)98011-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

HECT domain ubiquitin ligases (HECT E3s), typified by human E6AP and yeast Rsp5p, are unique among the several classes of known ubiquitin ligases in that they participate directly in the chemistry of substrate ubiquitination reactions. This chapter discusses strategies for the expression of active HECT E3s and the assays that are available for analyzing E2 interaction, ubiquitin-thioester formation, and substrate ubiquitination.

引用

页码：112 / 125

页数：14

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