Quantum dots as efficient energy acceptors in a time-resolved fluoroimmunoassay

被引：106

作者：

Hildebrandt, N

Charbonnière, LJ

Beck, M

Ziessel, RF

Löhmannsröben, HG

机构：

[1] Univ Potsdam, Inst Chem & Interdisziplinares Zentrum Photon, D-14476 Potsdam, Germany

[2] ECPM, ULP, UMR 7509, Lab Chim Mol, F-67087 Strasbourg, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 46期

关键词：

FRET (fluorescence resonance energy transfer); immunoassays; luminescence; quantum dots; terbium;

D O I：

10.1002/anie.200501552

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) On the dot: Efficient energy transfer from Tb complexes to quantum dots with an extremely large Förster radius of 81 Å is achieved by formation of a streptavidin - biotin coupled donor - acceptor complex. The long luminescence lifetime of the Tb donor and the high absorption of the quantum dots make this couple a powerful tool for analyzing biological and biochemical interactions. © 2005 Wiley-VCH Verlag GmbH &. Co. KGaA.

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页码：7612 / 7615

页数：4

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