The Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP) can function as a dominant suppressor of Bax-induced cell death of yeast

被引：35

作者：

Pan, L ^{[1
]}

Kawai, M ^{[1
]}

Yu, LH ^{[1
]}

Kim, KM ^{[1
]}

Hirata, A ^{[1
]}

Umeda, M ^{[1
]}

Uchimiya, H ^{[1
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

来源：

FEBS LETTERS | 2001年 / 508卷 / 03期

基金：

日本学术振兴会;

关键词：

Arabidopsis thaliana ethylene-responsive element binding protein; Bax; cell death; yeast; Arabidopsis;

D O I：

10.1016/S0014-5793(01)03098-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We identified genes based on screening of an Arabidopsis cDNA library for functional suppressors of mouse Bax-induced cell death of yeast cells. Interestingly, the cDNA encoding AtEBP, known as Arabidopsis thaliana ethylene-responsive element binding protein, was isolated numerous times in the functional screen (82% of all suppressors). Full-length AtEBP and its localization to the nucleus were essential for the suppression of Bax-induced cell death. Morphological abnormality of intracellular network that is a hallmark of Bax-induced cell death was attenuated by expression of AtEBP. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：375 / 378

页数：4

共 24 条

[1] Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein [J].

Buttner, M ;

Singh, KB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (11) :5961-5966

[2]

CAMP WV, 1990, P NATL ACAD SCI USA, V87, P9903

[3]

CAPELLI N, 1996, PLANT PHYSIOL, V112, P446

[4] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[5] Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression [J].

Fujimoto, SY ;

Ohta, M ;

Usui, A ;

Shinshi, H ;

Ohme-Takagi, M .

PLANT CELL, 2000, 12 (03) :393-404

[6]

GIETZ RD, 1994, MOL GENETICS YEAST P, P121

[7] Role of mitochondria and C-terminal membrane anchor of Bcl-2 in Bax induced growth arrest and mortality in Saccharomyces cerevisiae [J].

Greenhalf, W ;

Stephan, C ;

Chaudhuri, B .

FEBS LETTERS, 1996, 380 (1-2) :169-175

[8] Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly [J].

Haseloff, J ;

Siemering, KR ;

Prasher, DC ;

Hodge, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2122-2127

[9] WAVELENGTH MUTATIONS AND POSTTRANSLATIONAL AUTOXIDATION OF GREEN FLUORESCENT PROTEIN [J].

HEIM, R ;

PRASHER, DC ;

TSIEN, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12501-12504

[10] SEQUENCE REQUIREMENTS FOR NUCLEAR LOCATION OF SIMIAN VIRUS-40 LARGE-T-ANTIGEN [J].

KALDERON, D ;

RICHARDSON, WD ;

MARKHAM, AF ;

SMITH, AE .

NATURE, 1984, 311 (5981) :33-38

← 1 2 3 →