Uptake of a cholesterol-rich emulsion by neoplastic ovarian tissues

被引:55
作者
Ades, A [1 ]
Carvalho, JP
Graziani, SR
Amancio, RF
Souen, JS
Pinotti, JA
Maranhao, RC
机构
[1] Univ Sao Paulo, Med Sch Hosp, Dept Obstet & Gynecol, Fac Pharmaceut Sci, Sao Paulo, Brazil
[2] Univ Sao Paulo, Med Sch Hosp, Heart Inst INCOR, Fac Pharmaceut Sci, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
emulsions; ovarian cancer; lipoproteins; low-density lipoprotein receptors;
D O I
10.1006/gyno.2001.6203
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Objective. Previously, it was shown that a lipidic emulsion (LDE) composed of phospholipids and cholesterol esters which binds to low-density lipoprotein (LDL) receptors may concentrate in acute myeloid leukemia cells. In this study, we aimed to verify whether LDE also has the ability to concentrate in malignant ovarian cancer after being injected into the blood circulation of the patients. Methods. Three groups of women scheduled for surgery were included in the survey: 13 bearing malignant tumors, 9 with benign ovarian tumors, and 13 without ovarian tumor who were scheduled to undergo oophorectomy due to malignant disease of the uterine cervix or endometrium. On the day prior to surgery they were injected with LDE labeled with [C-14]cholesteryl oleate, Specimens of tumors and normal ovaries excised during surgery were lipid extracted and analyzed for radioactivity counting. Results were expressed in radioactive count (cpm) per gram of tissue. Results. The mean of the uptakes of the emulsion radioactivity by the malignant tumors was roughly eightfold greater when compared with that of the contralateral normal ovaries (2261 +/- 1444 and 275 +/- 137 cpm/g, respectively, P < 0.012), benign tumors, and normal ovaries of the patients without ovarian tumors. Conclusion. LDE has the ability to concentrate in malignant ovarian tumor tissue. Therefore, it can be used as a vehicle to direct cytotoxic drugs against malignant ovarian tumors, thus diminishing the side effects of chemotherapy, (C) 2001 Academic Press.
引用
收藏
页码:84 / 87
页数:4
相关论文
共 23 条
[1]  
FAN D, 1990, CANCER RES, V50, P3619
[2]   LOW-DENSITY LIPOPROTEIN AS A POTENTIAL VEHICLE FOR CHEMOTHERAPEUTIC-AGENTS AND RADIONUCLEOTIDES IN THE MANAGEMENT OF GYNECOLOGIC NEOPLASMS [J].
GAL, D ;
OHASHI, M ;
MACDONALD, PC ;
BUCHSBAUM, HJ ;
SIMPSON, ER .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1981, 139 (08) :877-885
[3]  
GINSBURG GS, 1982, J BIOL CHEM, V257, P8216
[4]   PHOTOIMMUNOTHERAPY AND BIODISTRIBUTION WITH AN OC125-CHLORIN IMMUNOCONJUGATE IN AN IN-VIVO MURINE OVARIAN-CANCER MODEL [J].
GOFF, BA ;
HERMANTO, U ;
RUMBAUGH, J ;
BLAKE, J ;
BAMBERG, M ;
HASAN, T .
BRITISH JOURNAL OF CANCER, 1994, 70 (03) :474-480
[5]   Effects of apolipoprotein B-100 on the metabolism of a lipid microemulsion model in rats [J].
Hirata, RDC ;
Hirata, MH ;
Mesquita, CH ;
Cesar, TB ;
Maranhao, RC .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1999, 1437 (01) :53-62
[6]  
HO YK, 1978, BLOOD, V52, P1099
[7]  
LESTAVELDELATTRE S, 1992, CANCER RES, V52, P3629
[8]  
LUNDBERG B, 1987, CANCER RES, V47, P4105
[9]  
LUNDBERG B, 1984, J LIPID RES, V25, P550
[10]  
MARANHAO RC, 1994, CANCER RES, V54, P4660