Crystallization and preliminary X-ray analysis of the inducible lysine decarboxylase from Escherichia coli

被引：9

作者：

Alexopoulos, Eftichia ^{[1
,2
]}

Kanjee, Usheer ^{[1
]}

Snider, Jamie ^{[1
]}

Houry, Walid A. ^{[1
]}

Pai, Emil F. ^{[1
,2
,3
]}

机构：

[1] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada

[2] Univ Toronto, Ontario Canc Inst, Div Canc Genom & Prote, Dept Med Biophys, Toronto, ON M5G 1L7, Canada

[3] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷

关键词：

D O I：

10.1107/S1744309108018757

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The decameric inducible lysine decarboxylase (LdcI) from Escherichia coli has been crystallized in space groups C2 and C222(1); the (TaBr122+)-Br-6 cluster was used to derivatize the C2 crystals. The method of single isomorphous replacement with anomalous scattering (SIRAS) as implemented in SHELXD was used to solve the Ta6Br122+-derivatized structure to 5 angstrom resolution. Many of the Ta6Br122+-binding sites had twofold and fivefold noncrystallographic symmetry. Taking advantage of this feature, phase modification was performed in DM. The electron-density map of LdcI displays many features in agreement with the low-resolution negative-stain electron-density map [Snider et al. ( 2006), J. Biol. Chem. 281, 1532-1546].

引用

页码：700 / 706

页数：7

共 30 条

[1] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[2] Phasing at high resolution using Ta6Br12 cluster [J].

Banumathi, S ;

Dauter, M ;

Dauter, Z .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2003, 59 :492-498

[3]

BOYD RF, 1995, BASIC MED MICROBIOLO

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5]

*BRUK AXS INC, 2000, SMART SAINT SADABS X

[6]

Cowtan K., 1994, JOINT CCP4 ESF EACBM, V31, P34

[7] Escherichia coli acid resistance:: Tales of an amateur acidophile [J].

Foster, JW .

NATURE REVIEWS MICROBIOLOGY, 2004, 2 (11) :898-907

[8] Solving a 300 kDa multimeric protein by low-resolution MAD phasing and averaging/phase extension [J].

Gomis-Rüth, FX ;

Coll, M .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2001, 57 :800-805

[9] ACID RESISTANCE IN ENTERIC BACTERIA [J].

GORDEN, J ;

SMALL, PLC .

INFECTION AND IMMUNITY, 1993, 61 (01) :364-367

[10] Arginine-agmatine antiporter in extreme acid resistance in Escherichia coli [J].

Iyer, R ;

Williams, C ;

Miller, C .

JOURNAL OF BACTERIOLOGY, 2003, 185 (22) :6556-6561

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