Fabrication of amperometric biosensors by entrapment of enzymes in functionalized polypyrrole films

被引：14

作者：

Cosnier, S ^{[1
]}

机构：

[1] Univ Grenoble 1, UMR CNRS 5630, Lab Electrochim Organ & Photochim Redox, F-38041 Grenoble 9, France

来源：

CANADIAN JOURNAL OF CHEMICAL ENGINEERING | 1998年 / 76卷 / 06期

关键词：

biosensors; electropolymerization; enzyme electrodes; polypyrrole; wired enzymes;

D O I：

10.1002/cjce.5450760605

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The concept and potentialities of an original electrochemical procedure of enzyme immobilization are described. This two-step method consists of the adsorption of an aqueous amphiphilic pyrrole monomer-enzyme mixture on an electrode surface followed by the electropolymerization of the adsorbed monomers. This procedure is compared with the conventional electrochemical methods of biosensor construction. Several bienzyme electrodes devoted to the improvement of the biosensor selectivity or to the amplification of the biosensor sensitivity are described. In addition, examples of organic phase enzyme electrode and the electrical wiring of immobilized enzymes are presented.

引用

页码：1000 / 1007

页数：8

共 51 条

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