West Nile virus in the endangered Spanish imperial eagle

被引:54
作者
Hoefle, Ursula [1 ,2 ]
Blanco, Juan M. [2 ]
Crespo, Elena [2 ]
Naranjo, Victoria [1 ]
Angel Jimenez-Clavero, Miguel [4 ]
Sanchez, Azucena [3 ]
de la Fuente, Jose [1 ,5 ]
Gortazar, Christian [1 ]
机构
[1] IREC CSIC UCLM JCCM, Inst Invest Recursos Cienget, Ciudad Real 13005, Spain
[2] CERI, Sevilleja De La Jara, Spain
[3] Cent Vet Lab, Madrid 28110, Spain
[4] CISA INIA, Madrid 28130, Spain
[5] Oklahoma State Univ, Dept Vet Pathobiol, Ctr Vet Hlth Sci, Stillwater, OK 74078 USA
关键词
Aquila adalberti; emerging disease; resident; Spain; Spanish imperial eagle; West Nile virus;
D O I
10.1016/j.vetmic.2007.11.006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Spanish imperial eagle (Aquila adalberti) is considered to be the most endangered European eagle. The species is an endemic resident in the Southwestern Iberian Peninsula. We used RT-PCR, immunohistochemistry and seroneutralization to test samples from 13 wild and 18 captive eagles. WNV was detected by RT-PCR in tissues and/or oropharyngeal swabs of eight of 10 (80%) imperial eagles analyzed, and both in apparently clinically healthy birds, and in animals that died due to secondary infections but had symptoms/lesions compatible with WNV. Immunohistochemistry detected WNV antigen in Purkinje cells in the cerebellum, epithelial cells of the gizzard and duodenum, perivascular inflammatory cells, and in Kupffer-cells and hepatocytes. Serum antibodies against WNV were detected in a total of five out of 21 imperial eagles (23.8%), including free-living nestlings (two out of nine samples, 22.2%) and captive adult eagles (three out of 12 samples, 25%). Our results evidence WNV circulation among free-living and captive Spanish imperial eagles in South-central Spain, a dry inland region with no previous WNV evidence, throughout 6 consecutive years. They also indicate the need for further research into this important zoonosis in order to better understand its epidemiology in the Mediterranean ecosystem and in order to understand the role of WNV in the population dynamics of the critically endangered Spanish imperial eagle. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:171 / 178
页数:8
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