Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides

被引：197

作者：

Gibson, Daniel G. ^{[1
]}

机构：

[1] J Craig Venter Inst, Synthet Biol Grp, Rockville, MD 20850 USA

来源：

NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 20期

关键词：

CHEMICAL GENE SYNTHESIS; RECOMBINATION CLONING; SELECTIVE ISOLATION; SYSTEMS BIOLOGY; COMPLEX GENOMES; MODEL SYSTEM; TRANSFORMATION; STRATEGIES; DESIGN; ACID;

D O I：

10.1093/nar/gkp687

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here it is demonstrated that the yeast Saccharomyces cerevisiae can take up and assemble at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded vector in one transformation event. These oligonucleotides can overlap by as few as 20 bp, and can be as long as 200 nucleotides in length. This straightforward scheme for assembling chemically-synthesized oligonucleotides could be a useful tool for building synthetic DNA molecules.

引用

页码：6984 / 6990

页数：7

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