A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum

被引：32

作者：

Casny, M

Rehder, D ^{[1
]}

Schmidt, H

Vilter, H

Conte, V

机构：

[1] Univ Hamburg, Inst Anorgan & Angew Chem, D-20146 Hamburg, Germany

[2] Umea Univ, Avdelningen Oorgan Kemi, S-90187 Umea, Sweden

[3] Univ Bari, Fac Agr 2, I-71100 Foggia, Italy

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2000年 / 80卷 / 1-2期

关键词：

bromoperoxidase; peroxovanadium; hydroperoxide; O-17; NMR;

D O I：

10.1016/S0162-0134(00)00024-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The O-17 NMR of bromoperoxidase in Tris buffer at pH 8 treated with O-17-enriched H2O2 reveals direct binding of peroxide to active site vanadium both in the symmetric, and asymmetric modes, the latter possibly due to hydroperoxide. In addition, non-active site HVO2(O-2)(2)(2-) is detected. The results are counter-checked with NMR data on peroxovanadium model compounds. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：157 / 160

页数：4

共 30 条

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[22] VANADATE-DEPENDENT BROMO IODOPEROXIDASE FROM ASCOPHYLLUM NODOSUM ALSO CONTAINS UNSPECIFIC LOW-AFFINITY BINDING-SITES FOR VANADATE(V) - A V-51 NMR INVESTIGATION, INCLUDING THE MODEL PEPTIDES PHE-GLU AND GLY-TYR [J].