Sucrose Phosphorylase Harbouring a Redesigned, Glycosyltransferase-Like Active Site Exhibits Retaining Glucosyl Transfer in the Absence of a Covalent Intermediate

被引：27

作者：

Goedl, Christiane ^{[1
]}

Nidetzky, Bernd ^{[1
]}

机构：

[1] Graz Univ Technol, Inst Biotechnol & Biochem Engn, A-8010 Graz, Austria

来源：

CHEMBIOCHEM | 2009年 / 10卷 / 14期

基金：

奥地利科学基金会;

关键词：

glycosides; glycosyltransferases; molecular mechanisms; phosphorylases; structure-activity relationships; ALPHA-GALACTOSYLTRANSFERASE; BASE CATALYSIS; MUTANT; MECHANISM; INSIGHTS; DONOR; STATE; ACID;

D O I：

10.1002/cbic.200900429

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Figure Presented) Mechanism makeover: We remodeled sucrose phosphorylase to mimic the active-site characteristics of a retaining glycosyltransferase. Thus, an enzymatic reaction coordinate for α-retaining glucosyl transfer through a covalent β-glucosyl enzyme intermediate was changed into one in which configurational retention appeared to be achieved through a direct front-side nucleophilic displacement reaction. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2333 / 2337

页数：5

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