Improved detection of Listeria monocytogenes in soft mould-ripened cheese

被引：19

作者：

Johansson, T

Ahola-Luttila, H

Pirhonen, T

Taimisto, AM

Haario, H

Laine, M

Salkinoja-Salonen, M

机构：

[1] Natl Vet & Food Res Inst, Dept Food Microbiol, FIN-00231 Helsinki, Finland

[2] Valio Ltd, Ctr Res & Dev, Helsinki, Finland

[3] Univ Helsinki, Dept Math, FIN-00014 Helsinki, Finland

[4] Univ Helsinki, Dept Appl Chem & Microbiol, FIN-00014 Helsinki, Finland

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2000年 / 88卷 / 05期

关键词：

D O I：

10.1046/j.1365-2672.2000.01030.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In comparison with standard methods, enrichment in half-Fraser broth for 24 h at 30 degrees C, followed by plating out onto Listeria monocytogenes blood agar (LMBA) and PALCAM medium combined with an additional streak proved to be the most rapid and specific method for the detection of indigenous L. monocytogenes populations from soft mould-ripened cheese, This procedure, with a high sensitivity (93%) and a low detection limit (1-10 cfu 25 g(-1)), provided negative and presumptive positive results within 2-3 d. Differences between LMBA, PALCAM and Oxford medium turned out to be highly significant (at 99% significance level); plating on LMBA after standard enrichment protocols giving the best overall results, An improvement in detection was also obtained by modifying the confirmation procedure. A loopful of culture (an additional streak) from PALCAM or Oxford medium was streaked on non-selective medium in addition to streaking only separate colonies as specified in the standards.

引用

页码：870 / 876

页数：7