Soybean agglutinin stimulated cholecystokinin release from cultured rabbit jejunal cells requires calcium influx via L-type calcium channels

被引：15

作者：

Jordinson, M ^{[1
]}

Beales, ILP ^{[1
]}

Calam, J ^{[1
]}

机构：

[1] Hammersmith Hosp, Imperial Coll Sch Med, Dept Gastroenterol, London W12 0NN, England

来源：

PEPTIDES | 1998年 / 19卷 / 09期

基金：

英国惠康基金;

关键词：

cholecystokinin; calcium; agglutinin; verapamil;

D O I：

10.1016/S0196-9781(98)00088-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the mechanism of soybran agglutinin (SBA) mediated cholecystokinin (CCK) release in enriched cultured cholecystokinin-secreting cells. 12-O-Tetradecanoylphorbol-13-acetate 1 mM significantly stimulated release of CCK-like-immunoreactivity (CCK-LI) by 55% +/- 17% (p < 0.05), which was blocked by the protein kinase C inhibitor staurosporine 100 nM. Forskolin 10 mM stimulated CCK-LI by 82% +/- 12% (p < 0.05) and this was inhibited by somatostatin 1 nM. 1-Phenylalanine 20 mM and Bay K 8644 1 mM stimulated CCK-LI by 69% +/- 22% and 60% +/- 19% respectively (I, < 0.05), these responses were completely abolished by the L-type calcium channel antagonist verapamil 10 mM. SEA 10 and 100 mu g/ml stimulated CCK-LI by 65% +/- 22% and 74% +/- 24% respectively (p < 0.05). The effect of SEA was inhibited by verapamil and N-acetylgalactosamine. We conclude that SBA stimulates CCK-LI through calcium flux via L-type calcium channels. (C) 1998 Elsevier Science Inc.

引用

页码：1541 / 1547

页数：7

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