Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates

被引：30

作者：

Lee, Jae-Seung

Seferos, Dwight S.

Giljohann, David A.

Mirkin, Chad A.

机构：

[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA

[2] Northwestern Univ, Int Inst Nanotechnol, Evanston, IL 60208 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 16期

关键词：

D O I：

10.1021/ja800797h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe the synthesis of small (2-nm diameter) gold nanoparticles densely functionalized with thiolated DNA (DNA-Au NPs) and a method to separate these particles from excess free DNA after synthesis. The separation method utilizes the thermodynamically enhanced binding properties of 2-nm DNA-Au NPs, compared to free excess DNA, to selectively hybridize these small particles to larger (15-nm diameter) DNA-Au NPs and form aggregates that can be isolated by simple centrifugation. These 2-nm DNA-Au NPs are obtained in a 46% overall yield, have a high surface coverage of DNA (64.8 +/- 6.4 pmol/cm(2)), and as a result, exhibit increased melting temperatures and cooperative melting properties.

引用

收藏

页码：5430 / +

页数：3

相关论文

共 35 条

[11] Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization [J].

He, L ;

Musick, MD ;

Nicewarner, SR ;

Salinas, FG ;

Benkovic, SJ ;

Natan, MJ ;

Keating, CD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (38) :9071-9077

[12] Alkanethiolate gold cluster molecules with core diameters from 1.5 to 5.2 nm: Core and monolayer properties as a function of core size [J].

Hostetler, MJ ;

Wingate, JE ;

Zhong, CJ ;

Harris, JE ;

Vachet, RW ;

Clark, MR ;

Londono, JD ;

Green, SJ ;

Stokes, JJ ;

Wignall, GD ;

Glish, GL ;

Porter, MD ;

Evans, ND ;

Murray, RW .

LANGMUIR, 1998, 14 (01) :17-30

[13] Synthesis of highly fluorescent gold nanoparticles for sensing Mercury(II) [J].

Huang, Chih-Ching ;

Yang, Zusing ;

Lee, Kun-Hong ;

Chang, Huan-Tsung .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (36) :6824-6828

[14] Maximizing DNA loading on a range of gold nanoparticle sizes [J].

Hurst, Sarah J. ;

Lytton-Jean, Abigail K. R. ;

Mirkin, Chad A. .

ANALYTICAL CHEMISTRY, 2006, 78 (24) :8313-8318

[15] What controls the melting properties of DNA-linked gold nanoparticle assemblies? [J].

Jin, RC ;

Wu, GS ;

Li, Z ;

Mirkin, CA ;

Schatz, GC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (06) :1643-1654

[16] Thioalkylated tetraethylene glycol: a new ligand for water soluble monolayer protected gold clusters [J].

Kanaras, AG ;

Kamounah, FS ;

Schaumburg, K ;

Kiely, CJ ;

Brust, M .

CHEMICAL COMMUNICATIONS, 2002, (20) :2294-2295

[17] Colorimetric detection of mercuric ion (Hg2+) in aqueous media using DNA-functionalized gold nanoparticles [J].

Lee, Jae-Seung ;

Han, Min Su ;

Mirkin, Chad A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (22) :4093-4096

[18] DNA-induced size-selective separation of mixtures of gold nanoparticles [J].

Lee, Jae-Seung ;

Stoeva, Savka I. ;

Mirkin, Chad A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (27) :8899-8903

[19] A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles [J].

Liu, JW ;

Lu, Y .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (22) :6642-6643

[20] A thermodynamic investigation into the binding properties of DNA functionalized gold nanoparticle probes and molecular fluorophore probes [J].

Lytton-Jean, AKR ;

Mirkin, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (37) :12754-12755

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