High-Quality Covalently Grafting Hemoglobin on Gold Electrodes: Characterization, Redox Thermodynamics and Bio-electrocatalysis

被引：3

作者：

Tian, Yuan ^{[1
]}

Ran, Qin ^{[1
]}

Xu, Jingjing ^{[1
]}

Xian, Yuezhong ^{[1
]}

Peng, Ru ^{[1
]}

Jin, Litong ^{[1
]}

机构：

[1] ECNU, Dept Chem, Shanghai 200062, Peoples R China

来源：

CHEMPHYSCHEM | 2009年 / 10卷 / 17期

基金：

中国国家自然科学基金;

关键词：

biocompatibility; carbodiimide reaction; click chemistry; electrochemistry; surface chemistry; SELF-ASSEMBLED MONOLAYERS; ACID-BASE EQUILIBRIA; BLUE-COPPER PROTEINS; CLICK CHEMISTRY; DIRECT ELECTROCHEMISTRY; HORSERADISH-PEROXIDASE; MATERIALS SCIENCE; CYTOCHROMES C; HEME-PROTEINS; POLYMER;

D O I：

10.1002/cphc.200900588

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Herein, we report a versatile surface chemistry methodology to covalently immobilize ligands proteins to self-assembled monolayers (SAMs) on gold electrode. The strategy is based on two steps: 1) the coupling of soluble azido PEG amimo ligand with an alkynl-terminated monolayer via click reaction and 2) covalent immobilization hemoglobin (Hb) to the amine-terminated ligand via carbodiimide reaction. Surface enhanced Raman scattering spectroscopy (SERS), atomic force microscopy (AFM), reflection absorption infrared spectrospy (RAIR) and cyclic voltammetry are used to characterize the model interfacial reactions. We also demonstrate the excellent biocompatibility of the interface for Hb immobilization and reliable application of the proposed method H2O2 biosensing. More-over the redox thermodynamics of the Fe3+/Fe2+ couple in Hb is also investigated.

引用

页码：3105 / 3111

页数：7

共 53 条

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