Proteomic Analysis of the Protective Effects of Platycodi Radix in Liver of Chronically Alcoholic Rats

被引：6

作者：

An, Jeung Hee ^{[2
]}

Kim, Dong-Sub ^{[1
]}

Lee, Yoo-Hyun ^{[1
]}

Ho, Jin-Nyoung ^{[1
]}

Kim, Hye-Kyung ^{[3
,4
]}

Kang, Ok-Ja ^{[5
]}

Shin, Il-Shik ^{[5
]}

Cho, Hong-Yon ^{[1
]}

机构：

[1] Korea Univ, Dept Food & Biotechnol, Coll Sci & Technol, Chungnam 339800, South Korea

[2] Sogang Univ, Dept Chem & Biomol Engn, Seoul, South Korea

[3] Hanseo Univ, Dept Food, Seosan, South Korea

[4] Hanseo Univ, Dept Biotechnol, Seosan, South Korea

[5] Kangnung Natl Univ, Fac Marine Biosci & Technol, Gangnung City, Gangwon, South Korea

来源：

JOURNAL OF MEDICINAL FOOD | 2009年 / 12卷 / 06期

关键词：

antioxidant; alcohol; liver disease; Platycodi Radix diet; proteomics; GLUTATHIONE-S-TRANSFERASE; OXIDATIVE STRESS; HEPG2; CELLS; MOUSE-LIVER; ETHANOL; GRANDIFLORUM; INHIBITION; ANTIOXIDANT; CONSUMPTION; EXPRESSION;

D O I：

10.1089/jmf.2009.0017

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In this study, we examined the effect of Platycodi Radix (PR) supplementation in chronically alcoholic rats. Sprague-Dawley rats were divided into three groups: control group (no alcohol), alcohol group (36.8% of total calories), and 0.3% PR group. The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were increased by alcohol treatment, and PR supplementation normalized the AST level. Moreover, alcohol-induced cytochrome P450 2E1 was decreased by PR treatment. Proteomic analysis of liver tissues of alcohol-exposed rats and PR-supplemented rats revealed that 50 different proteins functionally characterized as involved with cytoskeleton regulation, signal transduction, cytokine, apoptosis, and reactive oxygen species metabolism showed significant quantitative changes. The expression levels of glutathione Stransferase mu, Bcl-2-like protein, and peroxiredoxin IV were decreased in the alcoholic group, whereas the levels of these proteins were increased more than threefold in the PR group. However, the expression levels of smooth muscle actin, cytochrome P450 2D, mitogen-activated protein kinase 8, and 3 alpha-hydroxysteroid dehydrogenase were increased in the alcohol group and were decreased in the PR group. These data suggest that the antioxidant enzymes may play a protective role against alcohol-induced damage via oxidative stress defense mechanisms induced by PR supplementation.

引用

页码：1190 / 1198

页数：9

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