Optical detection of single molecules

被引：383

作者：

Nie, SM ^{[1
]}

Zare, RN ^{[1
]}

机构：

[1] STANFORD UNIV, DEPT CHEM, STANFORD, CA 94305 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 1997年 / 26卷

关键词：

fluorescence; confocal microscopy; near-field optics; DNA; molecular motors;

D O I：

10.1146/annurev.biophys.26.1.567

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in ultrasensitive instrumentation have allowed for the detection, identification, and dynamic studies of single molecules in the condensed phase. This measurement capability provides a new set of tools for scientists to address important current problems and to explore new frontiers in many scientific disciplines, such as chemistry, molecular biology, molecular medicine, and nanostructured materials. This review focuses on the methodologies and biological applications of single-molecule detection based on laser-induced fluorescence.

引用

页码：567 / 596

页数：30

共 153 条

[31] Biased diffusion, optical trapping, and manipulation of single molecules in solution
Chiu, DT
Zare, RN
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (27) : 6512 - 6513
[32] ANALYSIS OF NEAR-FIELD TIP PATTERNS INCLUDING OBJECT INTERACTION USING FINITE-DIFFERENCE TIME-DOMAIN CALCULATIONS
CHRISTENSEN, DA
[J]. ULTRAMICROSCOPY, 1995, 57 (2-3) : 189 - 195
[33] IN-VITRO SELECTION FROM PROTEIN AND PEPTIDE LIBRARIES
CLACKSON, T
WELLS, JA
[J]. TRENDS IN BIOTECHNOLOGY, 1994, 12 (05) : 173 - 184
[34] DNA: An extensible molecule
Cluzel, P
Lebrun, A
Heller, C
Lavery, R
Viovy, JL
Chatenay, D
Caron, F
[J]. SCIENCE, 1996, 271 (5250) : 792 - 794
[35] OBSERVATION OF INDIVIDUAL CHEMICAL REACTIONS IN SOLUTION
COLLINSON, MM
WIGHTMAN, RM
[J]. SCIENCE, 1995, 268 (5219) : 1883 - 1885
[36] Studies on single alkaline phosphatase molecules: Reaction rate and activation energy of a reaction catalyzed by a single molecule and the effect of thermal denaturation - The death of an enzyme
Craig, DB
Arriaga, EA
Wong, JCY
Lu, H
Dovichi, NJ
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (22) : 5245 - 5253
[37] Three-dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels
Dickson, RM
Norris, DJ
Tzeng, YL
Moerner, WE
[J]. SCIENCE, 1996, 274 (5289) : 966 - 969
[38] ATTOGRAM DETECTION LIMIT FOR AQUEOUS DYE SAMPLES BY LASER-INDUCED FLUORESCENCE
DOVICHI, NJ
MARTIN, JC
JETT, JH
KELLER, RA
[J]. SCIENCE, 1983, 219 (4586) : 845 - 847
[39] DNA-SEQUENCE DETERMINATION BY HYBRIDIZATION - A STRATEGY FOR EFFICIENT LARGE-SCALE SEQUENCING
DRMANAC, R
DRMANAC, S
STREZOSKA, Z
PAUNESKU, T
LABAT, I
ZEREMSKI, M
SNODDY, J
FUNKHOUSER, WK
KOOP, B
HOOD, L
CRKVENJAKOV, R
[J]. SCIENCE, 1993, 260 (5114) : 1649 - 1653
[40] NEAR-FIELD FLUORESCENCE IMAGING AND FLUORESCENCE LIFETIME MEASUREMENT OF LIGHT-HARVESTING COMPLEXES IN INTACT PHOTOSYNTHETIC MEMBRANES
DUNN, RC
HOLTOM, GR
METS, L
XIE, XS
[J]. JOURNAL OF PHYSICAL CHEMISTRY, 1994, 98 (12) : 3094 - 3098

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