Evaluation of an alkaline phosphatase-labeled oligonucleotide probe for the detection and enumeration of the thermostable-related hemolysin (trh) gene of Vibrio parahaemolyticus

被引：14

作者：

Nordstrom, Jessica L.

Rangdale, Rachel

Vickery, Michael C. L.

Phillips, Andrea M. B.

Murray, Shelley L.

Wagley, Sariqa

DePaola, Angelo

机构：

[1] US FDA, Gulf Coast Seafood Lab, Dauphin Isl, AL 36528 USA

[2] Cepheid, Sunnyvale, CA 94089 USA

[3] Univ So Mississippi, Gulf Coast Res Lab, Ocean Springs, MS USA

[4] Alaska Dept Environm Conservat, Anchorage, AK 99507 USA

来源：

JOURNAL OF FOOD PROTECTION | 2006年 / 69卷 / 11期

关键词：

D O I：

10.4315/0362-028X-69.11.2770

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom.

引用

收藏

页码：2770 / 2772

页数：3

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