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Evaluation of an alkaline phosphatase-labeled oligonucleotide probe for the detection and enumeration of the thermostable-related hemolysin (trh) gene of Vibrio parahaemolyticus

被引：14
作者：
Nordstrom, Jessica L.
Rangdale, Rachel
Vickery, Michael C. L.
Phillips, Andrea M. B.
Murray, Shelley L.
Wagley, Sariqa
DePaola, Angelo
机构：
[1] US FDA, Gulf Coast Seafood Lab, Dauphin Isl, AL 36528 USA
[2] Cepheid, Sunnyvale, CA 94089 USA
[3] Univ So Mississippi, Gulf Coast Res Lab, Ocean Springs, MS USA
[4] Alaska Dept Environm Conservat, Anchorage, AK 99507 USA
来源：
JOURNAL OF FOOD PROTECTION | 2006年 / 69卷 / 11期
关键词：
D O I：
10.4315/0362-028X-69.11.2770
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom.
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收藏
页码：2770 / 2772
页数：3
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