Voltammetric quantification of adenine and guanine at C60 modified glassy carbon electrodes

被引：15

作者：

Goyal, Rajendra N. ^{[1
]}

Singh, Sudhanshu P. ^{[1
]}

机构：

[1] Indian Inst Technol Roorkee, Dept Chem, Roorkee 247667, Uttar Pradesh, India

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2006年 / 6卷 / 12期

关键词：

voltammetry; adenine; guanine; DNA; fullerene-C-60;

D O I：

10.1166/jnn.2006.602

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Voltammetric determination of adenine and guanine has been carried out at C-60 modified glassy carbon electrode at physiological pH. Well-defined oxidation peaks were observed with Ep at similar to 990 mV and 692 mV for adenine and guanine respectively. Good colinearity was obtained in the concentration range 0.5 AM to 100.0 AM for adenine and guanine with sensitivity of similar to 0.06 mu A mu M-1 and similar to 0.03 mu A mu M-1, respectively. Simultaneous voltarnmetric determination of adenine and guanine has been described. Recovery studies for adenine and guanine in biological samples were also carried out. Interfering effect of some common metabolites including ascorbic acid has been evaluated. Analytical application of the developed protocol for determination of (G + C)/(A + T) ratio in DNA sample is also described.

引用

页码：3699 / 3704

页数：6

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