Kvα1 channels in murine arterioles:: differential cellular expression and regulation of diameter

被引:60
作者
Cheong, A [1 ]
Dedman, AM [1 ]
Xu, SZ [1 ]
Beech, DJ [1 ]
机构
[1] Univ Leeds, Sch Biomed Sci, Leeds LS2 9JT, W Yorkshire, England
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2001年 / 281卷 / 03期
关键词
K+ channel; endothelium; smooth muscle; vascular nerves; cerebral circulation; mouse;
D O I
10.1152/ajpheart.2001.281.3.H1057
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The primary objectives of this study were to reveal cell-specific expression patterns and functions of voltage-gated K+ channel (K(V)alpha1) subunits in precapillary arterioles of the murine cerebral circulation. K(V)alpha1 were detected using peptide-specific antibodies in immunofluorescence and Western blotting assays. K(V)1.2 was localized almost exclusively to endothelial cells, whereas K(V)1.5 was discretely localized to the nerves and nerve terminals that innervate the arterioles. K(V)1.5 also localized specifically to arteriolar nerves in human pial membrane. K(V)1.5 was notable for its absence from smooth muscle cells. K(V)1.3, K(V)1.4, and K(V)1.6 were localized to endothelial and smooth muscle cells, although K(V)1.4 had a low expression level. K(V)1.1 was not expressed. Therefore, we show that different cell types of pial arterioles have distinct physiological expression profiles of K(V)alpha1, conferring the possibility of differential modulation by extracellular and second messengers. Furthermore, we show recombinant agitoxin-2 and margatoxin are potent vasoconstrictors, suggesting that K(V)alpha1 subunits have a major function in determining arteriolar resistance to blood flow.
引用
收藏
页码:H1057 / H1065
页数:9
相关论文
共 44 条
[1]   Molecular identification of the role of voltage-gated K+ channels, Kv1.5 and Kv2.1, in hypoxic pulmonary vasoconstriction and control of resting membrane potential in rat pulmonary artery myocytes [J].
Archer, SL ;
Souil, E ;
Dinh-Xuan, AT ;
Schremmer, B ;
Mercier, JC ;
El Yaagoubi, A ;
Nguyen-Huu, L ;
Reeve, HL ;
Hampl, V .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (11) :2319-2330
[2]  
ATTALI B, 1993, J BIOL CHEM, V268, P24283
[3]   Identification, cloning and expression of rabbit vascular smooth muscle Kv1.5 and comparison with native delayed rectifier K+ current [J].
Clément-Chomienne, O ;
Ishii, K ;
Walsh, MP ;
Cole, WC .
JOURNAL OF PHYSIOLOGY-LONDON, 1999, 515 (03) :653-667
[4]   Molecular diversity of K+ channels [J].
Coetzee, WA ;
Amarillo, Y ;
Chiu, J ;
Chow, A ;
Lau, D ;
McCormack, T ;
Moreno, H ;
Nadal, MS ;
Ozaita, A ;
Pountney, D ;
Saganich, M ;
Vega-Saenz de Miera, E ;
Rudy, B .
MOLECULAR AND FUNCTIONAL DIVERSITY OF ION CHANNELS AND RECEPTORS, 1999, 868 :233-285
[5]   Mechanical stimulation regulates voltage-gated potassium currents in cardiac microvascular endothelial cells [J].
Fan, JP ;
Walsh, KB .
CIRCULATION RESEARCH, 1999, 84 (04) :451-457
[6]   Regulation of the cerebral circulation: Role of endothelium and potassium channels [J].
Faraci, FM ;
Heistad, DD .
PHYSIOLOGICAL REVIEWS, 1998, 78 (01) :53-97
[7]   PURIFICATION AND CHARACTERIZATION OF 3 INHIBITORS OF VOLTAGE-DEPENDENT K+ CHANNELS FROM LEIURUS-QUINQUESTRIATUS VAR HEBRAEUS VENOM [J].
GARCIA, ML ;
GARCIACALVO, M ;
HIDALGO, P ;
LEE, A ;
MACKINNON, R .
BIOCHEMISTRY, 1994, 33 (22) :6834-6839
[8]  
GARCIACALVO M, 1993, J BIOL CHEM, V268, P18866
[9]   TRANSFER OF THE SCORPION TOXIN RECEPTOR TO AN INSENSITIVE POTASSIUM CHANNEL [J].
GROSS, A ;
ABRAMSON, T ;
MACKINNON, R .
NEURON, 1994, 13 (04) :961-966
[10]   Positive and negative coupling of the endothelin ETA receptor to Ca2+-permeable channels in rabbit cerebral cortex arterioles [J].
Guibert, C ;
Beech, DJ .
JOURNAL OF PHYSIOLOGY-LONDON, 1999, 514 (03) :843-856