Utp9p Facilitates Msn5p-mediated Nuclear Reexport of Retrograded tRNAs in Saccharomyces cerevisiae

被引:27
作者
Eswara, Manoja B. K. [1 ]
McGuire, Andrew T. [1 ]
Pierce, Jacqueline B. [1 ]
Mangroo, Dev [1 ]
机构
[1] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词
POLYMERASE-III TRANSCRIPTION; CYTOPLASMIC TRANSFER-RNA; EXPORT MACHINERY; BINDING PROTEIN; TRANSPORT; AMINOACYLATION; IDENTIFICATION; COMPLEX; ACCUMULATION; COMPONENT;
D O I
10.1091/mbc.E09-06-0490
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Utp9p is a nucleolar protein that is part of a subcomplex containing several U3 snoRNA-associated proteins including Utp8p, which is a protein that shuttles aminoacyl-tRNAs from the nucleolus to the nuclear tRNA export receptors Los1p and Msn5p in Saccharomyces cerevisiae. Here we show that Utp9p is also an intranuclear component of the Msn5p-mediated nuclear tRNA export pathway. Depletion of Utp9p caused nuclear accumulation of mature tRNAs derived from intron-containing precursors, but not tRNAs made from intronless pre-tRNAs. Utp9p binds tRNA directly and saturably, and copurifies with Utp8p, Gsp1p, and Msn5p, but not with Los1p or aminoacyl-tRNA synthetases. Utp9p interacts directly with Utp8p, Gsp1p, and Msn5p in vitro. Furthermore, Gsp1p forms a complex with Msn5p and Utp9p in a tRNA-dependent manner. However, Utp9p does not shuttle between the nucleus and the cytoplasm. Because tRNA splicing occurs in the cytoplasm and the spliced tRNAs are retrograded back to the nucleus, we propose that Utp9p facilitates nuclear reexport of retrograded tRNAs. Moreover, the data suggest that Utp9p together with Utp8p translocate aminoacyl-tRNAs from the nucleolus to Msn5p and assist with formation of the Msn5p-tRNA-Gsp1p-GTP export complex.
引用
收藏
页码:5007 / 5025
页数:19
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