Toward a comprehensive temperature-sensitive mutant repository of the essential genes of Saccharomyces cerevisiae

被引：172

作者：

Ben-Aroya, Shay ^{[2
]}

Coombes, Candice ^{[1
]}

Kwok, Teresa ^{[2
]}

O'Donnell, Kathryn A. ^{[1
]}

Boeke, Jef D. ^{[1
]}

Hieter, Philip ^{[2
]}

机构：

[1] Johns Hopkins Univ, Sch Med, High Throughput Biol Ctr, Baltimore, MD 21205 USA

[2] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada

来源：

MOLECULAR CELL | 2008年 / 30卷 / 02期

关键词：

D O I：

10.1016/j.molcel.2008.02.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The Saccharomyces cereivisiae gene deletion project revealed that approximately 20% of yeast genes are required for viability. The analysis of essential genes traditionally relies on conditional mutants, typically temperature-sensitive (ts) alleles. We developed a systematic approach (termed "diploid shuffle") useful for generating a ts allele for each essential gene in S. cerevisiae and for improved genetic manipulation of mutant alleles; and gene constructs in general. Importantly, each ts allele resides at its normal genomic locus, flanked by specific cognate UPTAG and DNTAG bar codes. A subset of 250 ts mutants, including ts alleles for all uncharacterized essential genes and prioritized for genes with human counterparts, is now ready for distribution. The importance of this collection is demonstrated by biochemical and genetic screens that reveal essential genes involved in RNA processing and maintenance of chromosomal stability.

引用

页码：248 / 258

页数：11

共 55 条

[1]

ANDERSON DF, 2002, INT J COMMUTATIVE RI, V1, P11

[2]

Tackling an essential problem in functional proteomics of Saccharomyces cerevisiae -: art. no. 230 [J].

Aparicio, OM .

GENOME BIOLOGY, 2003, 4 (10)

[3]

The small-subunit processome is a ribosome assembly intermediate [J].

Bernstein, KA ;

Gallagher, JEG ;

Mitchell, BM ;

Granneman, S ;

Baserga, SJ .

EUKARYOTIC CELL, 2004, 3 (06) :1619-1626

[4]

The KEOPS complex: A Rosetta stone for telomere regulation? [J].

Bianchi, A ;

Shore, D .

CELL, 2006, 124 (06) :1125-1128

[5]

A genome-wide screen in Saccharomyces cerevisiae for genes affecting UV radiation sensitivity [J].

Birrell, GW ;

Giaever, G ;

Chu, AM ;

Davis, RW ;

Brown, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (22) :12608-12613

[6]

The PINc domain protein Utp24, a putative nuclease, is required for the early cleavage steps in 18S rRNA maturation [J].

Bleichert, Franziska ;

Grannemant, Sander ;

Osheim, Yvonne N. ;

Beyer, Ann L. ;

Baserga, Susan J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (25) :9464-9469

[7]

Exploring functional relationships between components of the gene expression machinery [J].

Burckin, T ;

Nagel, R ;

Mandel-Gutfreund, Y ;

Shiue, L ;

Clark, TA ;

Chong, JL ;

Chang, TH ;

Squazzo, S ;

Hartzog, G ;

Ares, M .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2005, 12 (02) :175-182

[8]

Prp43p is a DEAH-box spliceosome disassembly factor essential for ribosome biogenesis [J].

Combs, DJ ;

Nagel, RJ ;

Ares, M ;

Stevens, SW .

MOLECULAR AND CELLULAR BIOLOGY, 2006, 26 (02) :523-534

[9]

Heat-inducible degron and the making of conditional mutants [J].

Dohmen, RJ ;

Varshavsky, A .

UBIQUITIN AND PROTEIN DEGRADATION, PT B, 2005, 399 :799-822

[10]

Ribosome assembly in eukaryotes [J].

Fromont-Racine, M ;

Senger, B ;

Saveanu, C ;

Fasiolo, F .

GENE, 2003, 313 :17-42

← 1 2 3 4 5 6 →