Quantitative phase microscopy with asynchronous digital holography

被引:57
作者
Chalut, Kevin J. [1 ]
Brown, William J. [1 ]
Wax, Adam [1 ]
机构
[1] Duke Univ, Fitzpatrick Inst Photon, Durham, NC 27708 USA
关键词
D O I
10.1364/OE.15.003047
中图分类号
O43 [光学];
学科分类号
070207 [光学]; 0803 [光学工程];
摘要
We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acoustooptic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples. (c) 2007 Optical Society of America.
引用
收藏
页码:3047 / 3052
页数:6
相关论文
共 13 条
[1]
Digital holography for quantitative phase-contrast imaging [J].
Cuche, E ;
Bevilacqua, F ;
Depeursinge, C .
OPTICS LETTERS, 1999, 24 (05) :291-293
[2]
Ghiglia D. C., 1998, 2 DIMENSIONAL PHASE
[3]
Hahn S L., 1996, Hilbert Transforms in Signal Processing
[4]
Hilbert phase microscopy for investigating fast dynamics in transparent systems [J].
Ikeda, T ;
Popescu, G ;
Dasari, RR ;
Feld, MS .
OPTICS LETTERS, 2005, 30 (10) :1165-1167
[5]
DIGITAL HOLOGRAPHIC INTERFERENCE-PHASE MEASUREMENT USING THE FOURIER-TRANSFORM METHOD [J].
KREIS, T .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1986, 3 (06) :847-855
[6]
Kreis T., 1996, AKADEMIE VERLAG SERI, V1
[7]
Optical phase shifting with acousto-optic devices [J].
Li, EB ;
Yao, JQ ;
Yu, DY ;
Xi, JT ;
Chicharo, J .
OPTICS LETTERS, 2005, 30 (02) :189-191
[8]
Live cell refractometry using microfluidic devices [J].
Lue, Niyom ;
Popescu, Gabriel ;
Ikeda, Takahiro ;
Dasari, Ramachandra R. ;
Badizadegan, Kamran ;
Feld, Michael S. .
OPTICS LETTERS, 2006, 31 (18) :2759-2761
[9]
Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy [J].
Marquet, P ;
Rappaz, B ;
Magistretti, PJ ;
Cuche, E ;
Emery, Y ;
Colomb, T ;
Depeursinge, C .
OPTICS LETTERS, 2005, 30 (05) :468-470
[10]
Diffraction phase microscopy for quantifying cell structure and dynamics [J].
Popescu, G ;
Ikeda, T ;
Dasari, RR ;
Feld, MS .
OPTICS LETTERS, 2006, 31 (06) :775-777