Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23

被引：42

作者：

Boehm, MK

Perkins, SJ

机构：

[1] UCL, Royal Free & Univ Coll Med Sch, Dept Biochem & Mol Biol, London NW3 2PF, England

[2] UCL, Royal Free & Univ Coll Med Sch, Dept Oncol, London NW3 2PF, England

来源：

FEBS LETTERS | 2000年 / 475卷 / 01期

关键词：

antibody; carcinoembryonic antigen; homology model; structure prediction; scattering; single-chain Fv;

D O I：

10.1016/S0014-5793(00)01612-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

MFE23 is a single chain Fv antibody that has a high affinity for carcinoembryonic antigen (CEA), A full homology model for CEA based on V-type, I-type and C2-type immunoglobulin folds, 28 oligosaccharides and the interdomain angle of CD2 was validated using solution scattering data. The super-imposition of the intermolecular contacts observed in our recent crystal structure of MFE23 with the N-terminal domain of CEA permitted the MFE23-CEA complex to be modelled. Good surface and electrostatic complementarity and carbohydrate-unhindered access of MFE23 with the indentation between the first two CEA domains was observed. The model is supported by biochemical data and provides insight on the high affinity of MFE23 for CEA. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：11 / 16

页数：6

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