Nineteen compounds were isolated from the CHCl3 soluble portion of the heartwood of Eucalyptus citriodora. These compounds included trans-calamenene (1), T-muurolol (2), alpha-cadinol (3), 2 beta-hydroxy-alpha-cadinol (4), 4-hydroxy-3,5 -dimethoxybenzaldehyde (5), 4-hydroxy-3,5 -dimethoxybenzoic acid (6), linoleic acid (7), squalene (8), alpha-tocopherol(9), erythrodiol(10), morolic acid (11), betulonic acid (12), cycloeucalenol (13), cycloeucalenol vernolitate (14), beta-sitosterol (15), beta-sitosteryl-beta-D-glucopyranoside (16), beta-sitostenone (17), yangambin (18), sesamin (19). Among them, 14 is a new compound. The structures of these compounds were elucidated on the basis of spectroscopic evidence.