The concentrations of vitellogenin (vitellin) and protein in hemolymph, ovary and hepatopancreas in different ovarian stages of the freshwater prawn, Macrobrachium rosenbergii

The objectives were to measure the concentrations of vitellogenin (vitellin) and protein in hemolymph, ovary, and hepatopancreas of the freshwater prawn, Macrobrachium rosenbergii, in different stages of ovarian development. The ovarian development of M. rosenbergii was classified into five developmental stages (Stages I-V). Vitellogenin concentrations increased in the hemolymph of prawns in the early stages of ovarian development (Stage I or II) and were maintained at high levels until Stage V. There was no close association between ovarian development and the concentrations of circulating vitellogenin. Concentrations of protein in hemolymph and hepatopancreas remained constant during various stages of ovarian development. The ovarian stages closely correlated with the gonadosomatic index, the concentrations of ovarian vitellin and protein, respectively. Vitellogenin levels in hepatopancreas remained very low in different stages of the prawn although the highest levels were observed in Stage IV. No close association between hemolymph vitellogenin and ovarian vitellin was observed. The increase of vitellogenin concentration in hemolymph occurred earlier than vitellin content in ovary. (C) 1991 Elsevier Science Inc.