Isolation and identification of bluetongue virus

被引：79

作者：

Clavijo, A

Heckert, RA

Dulac, GC

Afshar, A

机构：

[1] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB R3E 3M4, Canada

[2] Univ Maryland, Virginia Maryland Reg Coll Vet Med, College Pk, MD 20742 USA

[3] Canadian Food Inspect Agcy, Anim Hlth & Prod Div, Nepean, ON K1A 0Y9, Canada

[4] Agr Canada, Anim Dis Res Inst, Nepean, ON K2H 8P9, Canada

来源：

JOURNAL OF VIROLOGICAL METHODS | 2000年 / 87卷 / 1-2期

关键词：

bluetongue virus; virus isolation; virus identification; immunoperoxidase assay; bluetonge diagnosis;

D O I：

10.1016/S0166-0934(00)00150-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bluetongue virus (BTV) is an arthropod-borne orbivirus that infects sheep, wild ruminants and occasionally cattle. Detection and specific identification of BTV is a multistep process. The first step involves the isolation of the virus from the animal's blood or other tissues, followed by inoculation of embryonating chicken eggs (ECE). After the virus has been amplified in ECE, it is passaged into BHK-21 cell culture for subsequent replication and identification. The virus is then amplified further and identified in microtiter plates by the immunoperoxidase assay using a group specific monoclonal antibody. Finally, the viral isolate is typed by a virus neutralization test. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：13 / 23

页数：11

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