The activation domain of the enhancer binding protein p45NF-E2 interacts with TAF(II)30 and mediates long-range activation of the alpha- and beta-globin gene loci in an erythroid cell line

被引:47
作者
Amrolia, PJ
Ramamurthy, L
Saluja, D
Tanese, N
Jane, SM
Cunningham, JM
机构
[1] ST JUDE CHILDRENS RES HOSP, DIV EXPT HEMATOL, MEMPHIS, TN 38101 USA
[2] NYU MED CTR, DEPT MICROBIOL, NEW YORK, NY 10016 USA
[3] ROYAL MELBOURNE HOSP, ROTARY BONE MARROW RES LAB, PARKVILLE, VIC 3050, AUSTRALIA
基金
英国惠康基金;
关键词
D O I
10.1073/pnas.94.19.10051
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have used the interaction between the erythroid-specific enhancer in hypersensitivity site 2 of the human beta-globin locus control region and the globin gene promoters as a paradigm to examine the mechanisms governing promoter/enhancer interactions in this locus, We have demonstrated that enhancer-dependent activation of the globin promoters is dependent on the presence of both a TATA box in the proximal promoter and the binding site for the erythroid-specific heteromeric transcription factor NF-E2 in the enhancer, Mutational analysis of the transcriptionally active component of NF-E2, p45NF-E2, localizes the critical region for this function to a proline-rich transcriptional activation domain in the NH2-terminal 80 amino acids of the protein. In contrast to the wild-type protein, expression of p45 NF-E2 lacking this activation domain in an NF-E2 null cell line fails to support enhancer-dependent transcription in transient assays. More significantly, the mutated protein also fails to reactivate expression of the endogenous beta- or alpha-globin loci in this cell line, Protein-protein interaction studies reveal that this domain of p45 NF-E2 binds specifically to a component of the transcription initiation complex, TATA binding protein associated factor TAF(II)130., These findings suggest one potential mechanism for direct recruitment of distal regulatory regions of the globin loci to the individual promoters.
引用
收藏
页码:10051 / 10056
页数:6
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