Assessment of genetic relationships among Pyrus species and cultivars using AFLP and RAPD markers

被引:94
作者
Monte-Corvo, L
Cabrita, L
Oliveira, C
Leitao, J [1 ]
机构
[1] Univ Algarve, UCTA, P-8000 Faro, Portugal
[2] Inst Super Agron, Seccao Hort, DPPA, P-1300 Lisbon, Portugal
关键词
AFLP; genetic similarity; molecular markers; pears; Pyrus; RAPD;
D O I
10.1023/A:1008794809807
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Twenty-five Pyrus communis L. cultivars including eight traditional Portuguese pears, and four commercial Pyrus pyrifolia (Burm.) Nak. (Japanese pear or 'nashi') cultivars were analysed by RAPD and AFLP techniques focusing on their molecular discrimination and the assessment of their genetic relatedness. Twenty-five primers generated 324 RAPD markers, among which 271 (84%) were polymorphic. The AFLP technique, using seven primer combinations, revealed a similar level of molecular polymorphisms (87%), representing 418 polymorphic bands among a total of 478 scored in autoradiographs. The high reproducibility of RAPD and AFLP techniques was confirmed comparing DNA samples from different extractions and different digestions of DNA from the same plant. Three genetic similarity matrices and respective dendrograms were elaborated on using RAPD, AFLP or joint RAPD and AFLP data. Both molecular marker techniques proved their reliability to assess genetic relationships among pear cultivars. P. pyrifolia cultivars exhibit a closer genetic relatedness, clustering apart from P. communis cultivars. Within P. communis, 'William's', as well as 'Doyenne du Comice', cluster close to their hybrids. Most of the Portuguese cultivars tend to cluster together, indicating to constitute a relatively independent genetic pool, which can be of interest in pear breeding programs.
引用
收藏
页码:257 / 265
页数:9
相关论文
共 21 条
[1]  
Bell RL, 1996, FRUIT BREEDING, P441
[2]  
Bell RL, 1990, GENETIC RESOURCES TE, P657
[3]   Identification of pear cultivars by molecular markers [J].
Botta, R ;
Akkak, A ;
Me, G ;
Radicati, L ;
Casavecchia, V .
SYMPOSIUM ON PLANT BIOTECHNOLOGY AS A TOOL FOR THE EXPLOITATION OF MOUNTAIN LANDS, 1998, (457) :63-70
[4]  
CEREZO M, 1989, ACTA HORTIC, V256, P111
[5]   Application of AFLP(TM)-based molecular markers to breeding of Populus spp [J].
Cervera, MT ;
Gusmao, J ;
Steenackers, M ;
VanGysel, A ;
VanMontagu, M ;
Boerjan, W .
PLANT GROWTH REGULATION, 1996, 20 (01) :47-52
[6]   Inheritance and linkage of isozyme loci in pear (Pyrus communis L) [J].
Chevreau, E ;
Leuliette, S ;
Gallet, M .
THEORETICAL AND APPLIED GENETICS, 1997, 94 (3-4) :498-506
[7]  
Chung Kyeong Ho, 1995, Journal of the Korean Society for Horticultural Science, V36, P829
[8]  
DAE IK, 1998, P 25 INT HORT C IHC, P447
[9]  
Doyle J. L. ., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362
[10]   Diversity of selected hop cultivars detected by fluorescent AFLPs [J].
Hartl, L ;
Seefelder, S .
THEORETICAL AND APPLIED GENETICS, 1998, 96 (01) :112-116