Identification of TRPM7 channels in human intestinal interstitial cells of Cajal

被引：28

作者：

Kim, Byung Joo ^{[3
]}

Park, Kyu Joo ^{[4
]}

Kim, Hyung Woo ^{[3
]}

Choi, Seok ^{[5
]}

Jun, Jae Yeoul ^{[5
]}

Chang, In Youb ^{[6
]}

Jeon, Ju-Hong ^{[7
,8
]}

So, Insuk ^{[7
,8
]}

Kim, Seon Jeong ^{[1
,2
]}

机构：

[1] Hanyang Univ, Ctr Bioartificial Muscle, Seoul 133791, South Korea

[2] Hanyang Univ, Dept Biomed Engn, Seoul 133791, South Korea

[3] Pusan Natl Univ, Sch Korean Med, Yangsan 626870, South Korea

[4] Seoul Natl Univ, Coll Med, Dept Surg, Seoul 110799, South Korea

[5] Chosun Univ, Coll Med, Dept Physiol, Kwangju 501759, South Korea

[6] Chosun Univ, Coll Med, Dept Anat, Kwangju 501759, South Korea

[7] Seoul Natl Univ, Coll Med, Ctr Bioartificial Muscle, Seoul 110799, South Korea

[8] Seoul Natl Univ, Coll Med, Dept Physiol, Seoul 110799, South Korea

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2009年 / 15卷 / 46期

关键词：

Electrophysiology; Interstitial cells of Cajal; TRPM cation channels; Transient receptor potential melastatin-type 7 protein; Human; Gastrointestinal tract; MURINE SMALL-INTESTINE; C-KIT; ELECTRICAL RHYTHMICITY; GASTROINTESTINAL-TRACT; PACEMAKER CURRENTS; GENERATE; PROLIFERATION; MECHANISM; STOMACH; CANCER;

D O I：

10.3748/wjg.15.5799

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

100201 [内科学];

摘要：

AIM: To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract. METHODS: Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs). RESULTS: The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La3+, TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION: These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves. (C) 2009 The WJG Press and Baishideng. All rights reserved.

引用

页码：5799 / 5804

页数：6

共 34 条

[1]

A key role for TRPM7 channels in anoxic neuronal death [J].