Bisphenol A induces superfeminization in the ramshorn snail Marisa cornuarietis (Gastropoda: Prosobranchia) at environmentally relevant concentrations

被引:143
作者
Oehlmann, Jorg
Schulte-Oehlmann, Ulrike
Bachmann, Jean
Oetken, Matthias
Lutz, Ilka
Kloas, Werner
Ternes, Thomas A.
机构
[1] Goethe Univ Frankfurt, Dept Ecol & Evolut, D-60054 Frankfurt, Germany
[2] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Inland Fisheries, Berlin, Germany
[3] Fed Inst Hydrol, Koblenz, Germany
关键词
bisphenol A; endocrine disruptor; hazard assessment; low-dose effects; Marisa cornuarietis; prosobranchia; reproductive toxicity; superfemale; temperature; xenoestrogen;
D O I
10.1289/ehp.8065
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Previous investigations have shown that bisphenol A (BPA) induces a superfeminization syndrome in the freshwater snail Marisa cornuarietis at concentrations as low as 1 mu g/L. Superfemales are characterized by the formation of additional female organs, enlarged accessory sex glands, gross malformations of the pallial oviduct, and a stimulation of egg and clutch production, resulting in increased female mortality. However, these studies were challenged on the basis of incomplete experimentation. Therefore, the objective of the current approach was to bridge several gaps in knowledge by conducting additional experiments. In an initial series of experiments, study results from the reproductive phase of the snails were evaluated in the sub-micrograms per liter range. Before and after the spawning season, superfemale responses were observed [NOEC (no observed effect concentration) 7.9 ng/L, EC(10) (effective concentration at 10%) 13.9 ng/L], which were absent during the spawning season. A further experiment investigated the temperature dependence of BPA responses by exposing snails at two temperatures in parallel. The adverse effect of BPA was at least partially masked at 27 degrees C (EC(10) 998 ng/L) when compared with 20 degrees C (EC10 14.8 ng/L). In M. cornuarietis, BPA acts as an estrogen receptor (ER) agonist, because effects were completely antagonized by a co-exposure to tamoxifen and Faslodex. Antiandrogenic effects of BPA, such as a significant decrease in penis length at 20 degrees C, were also observed. Competitive receptor displacement experiments indicate the presence of androgen- and estrogen-specific binding sites. The affinity for BPA of the estrogen binding sites in M. cornuarietis is higher than that of the ER in aquatic vertebrates. The results emphasize that prosobranchs are affected by BPA at lower concentrations than are other wildlife groups, and the findings also highlight the importance of exposure conditions.
引用
收藏
页码:127 / 133
页数:7
相关论文
共 44 条
[21]   Amphibians as a model to study endocrine disruptors: II. Estrogenic activity of environmental chemicals in vitro and in vivo [J].
Kloas, W ;
Lutz, I ;
Einspanier, R .
SCIENCE OF THE TOTAL ENVIRONMENT, 1999, 225 (1-2) :59-68
[22]   Effects of nonylphenol, bisphenol A, and their mixture on the viviparous swordtail fish (Xiphophorus helleri) [J].
Kwak, HI ;
Bae, MO ;
Lee, MH ;
Lee, YS ;
Lee, BJ ;
Kang, KS ;
Chae, CH ;
Sung, HJ ;
Shin, JS ;
Kim, JH ;
Mar, WC ;
Sheen, YY ;
Cho, MH .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2001, 20 (04) :787-795
[23]   Antiandrogenic effects of bisphenol A and nonylphenol on the function of androgen receptor [J].
Lee, HJ ;
Chattopadhyay, S ;
Gong, EY ;
Ahn, RS ;
Lee, K .
TOXICOLOGICAL SCIENCES, 2003, 75 (01) :40-46
[24]   Bisphenol A induces feminization in Xenopus laevis tadpoles [J].
Levy, G ;
Lutz, I ;
Krüger, A ;
Kloas, W .
ENVIRONMENTAL RESEARCH, 2004, 94 (01) :102-111
[25]   Amphibians as a model to study endocrine disruptors: I. Environmental pollution and estrogen receptor binding [J].
Lutz, I ;
Kloas, W .
SCIENCE OF THE TOTAL ENVIRONMENT, 1999, 225 (1-2) :49-57
[26]   Estrogenic compounds affect development of harpacticoid copepod Tigriopus japonicus [J].
Marcial, HS ;
Hagiwara, A ;
Snell, TW .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2003, 22 (12) :3025-3030
[27]   In vitro and in vivo interactions of bisphenol A and its metabolite, bisphenol A glucuronide, with estrogen receptors α and β [J].
Matthews, JB ;
Twomey, K ;
Zacharewski, TR .
CHEMICAL RESEARCH IN TOXICOLOGY, 2001, 14 (02) :149-157
[28]   Critical appraisal of the evidence for tributyltin-mediated endocrine disruption in mollusks [J].
Matthiessen, P ;
Gibbs, PE .
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 1998, 17 (01) :37-43
[29]  
Metcalfe CD, 2001, ENVIRON TOXICOL CHEM, V20, P297, DOI [10.1897/1551-5028(2001)020&lt
[30]  
0297:EPOCDI&gt