Effect of puerarin on P2X3 receptor involved in hyperalgesia after burn injury in the rat

被引：30

作者：

Xu, Changshui ^{[1
]}

Li, Guilin ^{[1
]}

Gao, Yun ^{[1
]}

Liu, Shuangmei ^{[1
]}

Lin, Jiari ^{[1
]}

Zhang, Jun ^{[1
]}

Li, Xin ^{[1
]}

Liu, Han ^{[1
]}

Liang, Shangdong ^{[1
]}

机构：

[1] Nanchang Univ, Coll Med, Dept Physiol, Nanchang 330006, Jiangxi, Peoples R China

来源：

BRAIN RESEARCH BULLETIN | 2009年 / 80卷 / 06期

基金：

中国国家自然科学基金;

关键词：

P2X(3) receptor; Puerarin; Burn injury pain; Hyperalgesia; ROOT GANGLION NEURONS; SENSORY NEURONS; NEUROPATHIC PAIN; ATP RELEASE; CONSTRICTION INJURY; ENDOGENOUS ATP; SKIN; NOCICEPTORS; ACTIVATION; MECHANISMS;

D O I：

10.1016/j.brainresbull.2009.08.027

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The study investigated the effects of puerarin on P2X(3) receptor involved in hyperalgesia after burn injury in the rat. Superficial second degree burn injury models were adopted. Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured and the P2X(3) receptor expressions in dorsal root ganglion (DRG) from burn injury models rats were detected by immunohistochemistry, in situ hybridization, RT-PCR and western blot. MML and TWL in untreated superficial second paw burn rats were reduced. MWL and TWL of puerarin-treated superficial second paw burn rats showed significant increase compared with untreated superficial second paw burn rats. Puerarin can decrease the hyperalgesia after burn injury. At day 3 post-burn, the expressions of P2X(3) protein and mRNA in DRG neurons in untreated superficial second degree back burn group were increased significantly compared with sham back burn group, puerarin-treated back unburned control group, blank back control group,while in puerarin-treated superficial second degree back burn group, the P2X(3) protein and mRNA expressions were decreased markedly. There is no significant difference in sham back burn group, puerarin-treated back unburned control group, blank back control group. Therefore, puerarin may reduce the nociceptive transmission of burn injury pain mediated by P2X(3) receptor and alleviate P2X(3) receptor involved in hyperalgesia after burn injury in the rats. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：341 / 346

页数：6

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