Short-chain fatty acids act as antiinflammatory mediators by regulating prostaglandin E2 and cytokines

被引：279

作者：

Cox, Mary Ann ^{[2
]}

Jackson, James ^{[1
]}

Stanton, Michaela ^{[1
]}

Rojas-Triana, Alberto ^{[1
]}

Bober, Loretta ^{[1
]}

Laverty, Maureen ^{[3
]}

Yang, Xiaoxin ^{[3
]}

Zhu, Feng ^{[3
]}

Liu, Jianjun ^{[3
]}

Wang, Suke ^{[3
]}

Monsma, Frederick ^{[3
]}

Vassileva, Galya ^{[3
]}

Maguire, Maureen ^{[3
]}

Gustafson, Eric ^{[3
]}

Bayne, Marvin ^{[1
,3
]}

Chou, Chuan-Chu ^{[1
]}

Lundell, Daniel ^{[4
]}

Jenh, Chung-Her ^{[1
]}

机构：

[1] Schering Plough Corp, Res Inst, Dept Cardiovasc & Metab Dis Res, Kenilworth, NJ 07033 USA

[2] Schering Plough Corp, Res Inst, Dept Tumor Biol, Kenilworth, NJ 07033 USA

[3] Schering Plough Corp, Res Inst, Dept Discovery Technol, Kenilworth, NJ 07033 USA

[4] Schering Plough Corp, Res Inst, Dept Inflammat, Kenilworth, NJ 07033 USA

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2009年 / 15卷 / 44期

关键词：

Short-chain fatty acids; GPR43; GPR41; Human monocytes; Prostaglandin E-2; Chemokines; Cytokines; PROTEIN-COUPLED RECEPTORS; NF-KAPPA-B; HUMAN-NEUTROPHILS; FUNCTIONAL-CHARACTERIZATION; ENDOTHELIAL-CELLS; CARBOXYLIC-ACIDS; ACTIVATION; COLITIS; BUTYRATE; GPR43;

D O I：

10.3748/wjg.15.5549

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To investigate the effect of short-chain fatty acids (SCFAs) on production of prostaglandin E-2 (PGE(2)), cytokines and chemokines in human monocytes. METHODS: Human neutrophils and monocytes were isolated from human whole blood by using 1-Step Polymorph and RosetteSep Human Monocyte Enrichment Cocktail, respectively. Human GPR41 and GPR43 mRNA expression was examined by quantitative real-time polymerase chain reaction. The calcium flux assay was used to examine the biological activities of SCFAs in human neutrophils and monocytes. The effect of SCFAs on human monocytes and peripheral blood mononuclear cells (PBMC) was studied by measuring PGE(2), cytokines and chemokines in the supernatant. The effect of SCFAs in vivo was examined by intraplantar injection into rat paws. RESULTS: Human GPR43 is highly expressed in human neutrophils and monocytes. SCFAs induce robust calcium flux in human neutrophils, but not in human monocytes. In this study, we show that SCFAs can induce human monocyte release of PGE(2) and that this effect can be enhanced in the presence of lipopolysaccharide (LPS). In addition, we demonstrate that PGE(2) production induced by SCFA was inhibited by pertussis toxin, suggesting the involvement of a receptor-mediated mechanism. Furthermore, SCFAs can specifically inhibit constitutive monocyte chemotactic protein-1 (MCP-1) production and LPS-induced interleukin-10 (IL-10) production in human monocytes without affecting the secretion of other cytokines and chemokines examined. Similar activities were observed in human PBMC for the release of PGE2, MCP-1 and IL-10 after SCFA treatment. In addition, SCFAs inhibit LPS-induced production of tumor necrosis factor-alpha and interferon-gamma in human PBMC. Finally, we show that SCFAs and LPS can induce PGE(2) production in vivo by intraplantar injection into rat paws (P < 0.01). CONCLUSION: SCFAs can have distinct antiinflammatory activities due to their regulation of PGE(2), cytokine and chemokine release from human immune cells. (C) 2009 The WJG Press and Baishideng. All rights reserved.

引用

页码：5549 / 5557

页数：9

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