Detection of aac(6′)-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel

被引:33
作者
Chmelnitsky, Inna [1 ]
Hermesh, Orit [1 ]
Navon-Venezia, Shiri [1 ]
Strahilevitz, Jacob [2 ]
Carmeli, Yehuda [1 ]
机构
[1] Tel Aviv Med Ctr & Sch Med, Div Epidemiol, Tel Aviv, Israel
[2] Hadassah Hebrew Univ, Dept Clin Microbiol & Infect Dis, Jerusalem, Israel
基金
以色列科学基金会;
关键词
beta-lactamases; carbapenem resistance; quinolone resistance; plasmids; MEDIATED QUINOLONE RESISTANCE; TRANSFERABLE PLASMID; GENE;
D O I
10.1093/jac/dkp272
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: We aimed to evaluate the occurrence and characteristics of plasmid-mediated quinolone resistance (PMQR) genes in KPC-producing (KPC-P) Klebsiella pneumoniae (Kpn) isolates in Tel Aviv Medical Center, Israel. Methods: Forty-seven KPC-P Kpn isolates were studied. Antibiotic susceptibilities were determined by Vitek 2, Etest or agar dilution. beta-Lactamases and PMOR determinants were detected by PCR. For plasmid characterization, transformation, transconjugation, restriction mapping and Southern blot analysis were performed . Results: Six out of 47 (13%) KPC-P isolates carried aac(6')-Ib-cr. Acquisition of aac(6')-Ib-cr-encoding plasmids increased the MIC of ciprofloxacin by 2-fold. In five of six KPC-P isolates, aac(6')-Ib-cr and bla(KPC-2) were encoded on the same plasmid. Conclusions: The most prevalent PMQR gene in the studied KPC-P X pneumoniae isolates is aac(6')-Ib-cr. The co-existence of PMQR genes with KPC on the same plasmid poses a serious epidemiological, clinical and public-health threat.
引用
收藏
页码:718 / 722
页数:5
相关论文
共 13 条
  • [1] qnrD, a Novel Gene Conferring Transferable Quinolone Resistance in Salmonella enterica Serovar Kentucky and Bovismorbificans Strains of Human Origin
    Cavaco, L. M.
    Hasman, H.
    Xia, S.
    Aarestrup, F. M.
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (02) : 603 - 608
  • [2] Plasmid-Mediated qnrB2 and carbapenemase gene blaKPC-2 carried on the same plasmid in carbapenem-resistant ciprofloxacin-susceptible Enterobacter cloacae isolates
    Chmelnitsky, Inna
    Navon-Venezia, Shiri
    Strahilevitz, Jacob
    Carmeli, Yehuda
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (08) : 2962 - 2965
  • [3] Clinical and Laboratory Standards Institute, 2009, M100S19 CLSI S
  • [4] Cloning of a novel gene for quinolone resistance from a transferable plasmid in Shigella flexneti 2b
    Hata, M
    Suzuki, M
    Matsumoto, M
    Takahashi, M
    Sato, K
    Ibe, S
    Sakae, K
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (02) : 801 - 803
  • [5] qnrB, another plasmid-mediated gene for quinolone resistance
    Jacoby, GA
    Walsh, KE
    Mills, DM
    Walker, VJ
    Oh, H
    Robicsek, A
    Hooper, DC
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (04) : 1178 - 1182
  • [6] A novel large plasmid carrying multiple β-lactam resistance genes isolated from a Klebsiella pneumoniae strain
    Li, L
    Lim, CK
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (06) : 1038 - 1048
  • [7] Quinolone resistance from a transferable plasmid
    Martínez-Martínez, L
    Pascual, A
    Jacoby, GA
    [J]. LANCET, 1998, 351 (9105) : 797 - 799
  • [8] Interaction of plasmid and host quinolone resistance
    Martínez-Martínez, L
    Pascual, A
    García, I
    Tran, J
    Jacoby, GA
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2003, 51 (04) : 1037 - 1039
  • [9] Plasmid-mediated quinolone resistance
    Martinez-Martinez, Luis
    Cano, Maria Eliecer
    Rodriguez-Martinez, Jose Manuel
    Calvo, Jorge
    Pascual, Alvaro
    [J]. EXPERT REVIEW OF ANTI-INFECTIVE THERAPY, 2008, 6 (05) : 685 - 711
  • [10] Transferable resistance to aminoglycosides by methylation of G1405 in 16S rRNA and to hydrophilic fluoroquinolones by QepA-mediated efflux in Escherichia coli
    Perichon, Bruno
    Courvalin, Patrice
    Galimand, Marc
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (07) : 2464 - 2469