AUT03DEM - an automated and high throughput program for image reconstruction of icosahedral particles

被引：162

作者：

Yan, Xiaodong

Sinkovits, Robert S.

Baker, Timothy S. ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Dept Biol Mol, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, San Diego Supercomp Ctr, La Jolla, CA 92093 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2007年 / 157卷 / 01期

关键词：

3D image reconstruction; icosahedral virus; origin and orientation determination; electron cryo-microscopy; automated 3D image reconstruction; random model reconstruction; traditional model reconstruction; unbiased model reconstruction; image processing software;

D O I：

10.1016/j.jsb.2006.08.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

AUTO3DEM is all automation system designed to accelerate the cornputationally intensive process of three-dimensional structure determination from images of vitrified icosahedral virus particles. With minimal user input and intervention, AUTO3DEM manages the flow of data between the major image reconstruction programs, monitors the progress of the computations, and intelligently updates the input parameters Lis the resolution of the model is improved. It is designed to be used on any computer running the Linux or UNIX operating systems and call be run in parallel mode on multi-processor systems. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：73 / 82

页数：10

共 16 条

[1] Adding the third dimension to virus life cycles: Three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs [J].