Cryopreservation of mouse spermatozoa

被引：170

作者：

Nakagata, N ^{[1
]}

机构：

[1] Kumamoto Univ, Ctr Anim Resources & Dev, Div Reprod Engn, Kumamoto 8600811, Japan

来源：

MAMMALIAN GENOME | 2000年 / 11卷 / 07期

关键词：

D O I：

10.1007/s003350010109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Recently, it has become possible to freeze a large number of mouse spermatozoa immediately after collection from the epididymides of a small number of males. The cryopreservation of spermatozoa is simpler, less time-consuming, and less costly than that of embryos for maintaining various strains of mice with induced mutations. This chapter attempts to provide a realistic overview of the cryopreservation of mouse spermatozoa and to describe a detailed procedure for mouse sperm freezing.

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收藏

页码：572 / 576

页数：5

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