Proteomic analysis of cervical-vaginal fluid: Identification of novel biomarkers for detection of intra-amniotic infection

被引：66

作者：

Gravett, Michael G.

Thomas, Archana

Schneider, Kimberly A.

Reddy, Ashok P.

Dasari, Surendra

Jacob, Thomas

Lu, Xinfang

Rodland, Matthew

Pereira, Leonardo

Sadowsky, Drew W.

Roberts, Charles T., Jr.

Novy, Miles J.

Nagalla, Srinivasa R.

机构：

[1] Univ Washington, Dept Obstet & Gynecol, Seattle, WA 98195 USA

[2] Oregon Natl Primate Res Ctr, Div Reprod Sci, Beaverton, OR 97006 USA

[3] Oregon Hlth & Sci Univ, Dept Obstet & Gynecol, Portland, OR 97239 USA

[4] Oregon Hlth & Sci Univ, Dept Pediat, Portland, OR 97239 USA

[5] ProteoGenix Inc, Portland, OR 97213 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 01期

关键词：

intra-amniotic infection; preterm birth; cervical-vaginal fluid;

D O I：

10.1021/pr060149v

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Intra-amniotic infection (IAI) is associated with preterm birth and perinatal mortality. To identify potential biomarkers, we performed a comprehensive survey of the cervical-vaginal fluid (CVF) proteome from a primate IAI model utilizing multidimensional protein identification technology (LC/LC-MS/MS) and MALDI-TOF-MS analyses. Analyses of CVF proteome identified 205 unique proteins and differential expression of 27 proteins in controls and IAI samples. Protein expression signatures and immunodetection of specific biomarkers identified can be employed for noninvasive detection of IAI.

引用

收藏

页码：89 / 96

页数：8

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