Cytogenetic analysis of human blastocysts

被引:43
作者
Clouston, HJ
Herbert, M
Fenwick, J
Murdoch, AP
Wolstenholme, J
机构
[1] Int Ctr Life, Inst Human Genet, Dept Cytogenet, Newcastle Upon Tyne NE1 3BZ, Tyne & Wear, England
[2] Int Ctr Life, Newcastle Upon Tyne, Tyne & Wear, England
关键词
blastocyst; karyotype; aneuploidy; mosaicism;
D O I
10.1002/pd.502
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Objectives To investigate the range and incidence of chromosome abnormalities in a large series of human blastocysts, using classic cytogenetic techniques. Methods Using thymidine, cell division is synchronized in spare five-to-eight-day-old human blastocysts generated by IVF. A simple acetic acid disaggregation step produces discrete metaphases for G-band analysis. Subsequent FISH analysis of both metaphase and interphase nuclei allows further exploration of an abnormality detected by G-banding, including the investigation of any mosaicism. Results A total of 438 blastocysts have been prepared. Where analysis was possible, 3% appeared polyploid (mainly tetraploid), 29% were diploid: tetraploid mosaics and 68% were uniformly diploid. Abnormalities observed include triploidy, trisomy 16, trisomy 2, trisomy for unidentifiable D-group chromosome, mosaic trisomy 3, and mosaic trisomy 3 and trisomy 7. Conclusion Comparison of results with existing data from both first trimester pregnancies and cleavage stage embryos suggests significant loss of haploid and monosomic embryos, as well as loss of some trisomies, prior to the blastocyst stage. It appears that the general range and incidence of most main groups of constitutional abnormalities observed in the first trimester (including mosaic forms) are in place by the blastocyst stage. Copyright (C) 2002 John Wiley Sons, Ltd.
引用
收藏
页码:1143 / 1152
页数:10
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