The Tasmanian Devil Transcriptome Reveals Schwann Cell Origins of a Clonally Transmissible Cancer

被引：168

作者：

Murchison, Elizabeth P. ^{[1
,2
,3
]}

Tovar, Cesar ^{[4
]}

Hsu, Arthur ^{[5
]}

Bender, Hannah S. ^{[1
,2
,3
]}

Kheradpour, Pouya ^{[6
]}

Rebbeck, Clare A. ^{[1
]}

Obendorf, David ^{[4
]}

Conlan, Carly ^{[1
]}

Bahlo, Melanie ^{[5
]}

Blizzard, Catherine A. ^{[4
]}

Pyecroft, Stephen ^{[7
]}

Kreiss, Alexandre ^{[4
]}

Kellis, Manolis ^{[6
,8
,9
]}

Stark, Alexander ^{[6
,8
,9
]}

Harkins, Timothy T. ^{[10
]}

Graves, Jennifer A. Marshall ^{[2
,3
]}

Woods, Gregory M. ^{[4
]}

Hannon, Gregory J. ^{[1
]}

Papenfuss, Anthony T. ^{[5
]}

机构：

[1] Cold Spring Harbor Lab, Howard Hughes Med Inst, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA

[2] Australian Natl Univ, Res Sch Biol, Canberra, ACT 0200, Australia

[3] Australian Natl Univ, ARC Ctr Excellence Kangaroo Genom, Canberra, ACT 0200, Australia

[4] Univ Tasmania, Menzies Res Inst, Hobart, Tas 7000, Australia

[5] Walter & Eliza Hall Inst Med Res, Bioinformat Div, Melbourne, Vic 3052, Australia

[6] MIT, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA

[7] Dept Primary Ind & Water, Mt Pleasant Anim Hlth Labs, Kings Meadows, Tas 7249, Australia

[8] MIT, Broad Inst, Cambridge, MA 02141 USA

[9] Harvard Univ, Cambridge, MA 02141 USA

[10] Roche Appl Sci, Indianapolis, IN 46250 USA

来源：

SCIENCE | 2010年 / 327卷 / 5961期

基金：

奥地利科学基金会;

关键词：

FACIAL-TUMOR DISEASE; SARCOPHILUS-HARRISII; IMMUNOHISTOCHEMICAL CHARACTERIZATION; VENEREAL TUMOR; MICRORNAS; DIVERSITY; NEOPLASMS; EVOLUTION; PROTEIN; SYSTEM;

D O I：

10.1126/science.1180616

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Tasmanian devil, a marsupial carnivore, is endangered because of the emergence of a transmissible cancer known as devil facial tumor disease (DFTD). This fatal cancer is clonally derived and is an allograft transmitted between devils by biting. We performed a large-scale genetic analysis of DFTD with microsatellite genotyping, a mitochondrial genome analysis, and deep sequencing of the DFTD transcriptome and microRNAs. These studies confirm that DFTD is a monophyletic clonally transmissible tumor and suggest that the disease is of Schwann cell origin. On the basis of these results, we have generated a diagnostic marker for DFTD and identify a suite of genes relevant to DFTD pathology and transmission. We provide a genomic data set for the Tasmanian devil that is applicable to cancer diagnosis, disease evolution, and conservation biology.

引用

页码：84 / 87

页数：4

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