The Tasmanian Devil Transcriptome Reveals Schwann Cell Origins of a Clonally Transmissible Cancer

被引:168
作者
Murchison, Elizabeth P. [1 ,2 ,3 ]
Tovar, Cesar [4 ]
Hsu, Arthur [5 ]
Bender, Hannah S. [1 ,2 ,3 ]
Kheradpour, Pouya [6 ]
Rebbeck, Clare A. [1 ]
Obendorf, David [4 ]
Conlan, Carly [1 ]
Bahlo, Melanie [5 ]
Blizzard, Catherine A. [4 ]
Pyecroft, Stephen [7 ]
Kreiss, Alexandre [4 ]
Kellis, Manolis [6 ,8 ,9 ]
Stark, Alexander [6 ,8 ,9 ]
Harkins, Timothy T. [10 ]
Graves, Jennifer A. Marshall [2 ,3 ]
Woods, Gregory M. [4 ]
Hannon, Gregory J. [1 ]
Papenfuss, Anthony T. [5 ]
机构
[1] Cold Spring Harbor Lab, Howard Hughes Med Inst, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA
[2] Australian Natl Univ, Res Sch Biol, Canberra, ACT 0200, Australia
[3] Australian Natl Univ, ARC Ctr Excellence Kangaroo Genom, Canberra, ACT 0200, Australia
[4] Univ Tasmania, Menzies Res Inst, Hobart, Tas 7000, Australia
[5] Walter & Eliza Hall Inst Med Res, Bioinformat Div, Melbourne, Vic 3052, Australia
[6] MIT, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA
[7] Dept Primary Ind & Water, Mt Pleasant Anim Hlth Labs, Kings Meadows, Tas 7249, Australia
[8] MIT, Broad Inst, Cambridge, MA 02141 USA
[9] Harvard Univ, Cambridge, MA 02141 USA
[10] Roche Appl Sci, Indianapolis, IN 46250 USA
基金
奥地利科学基金会;
关键词
FACIAL-TUMOR DISEASE; SARCOPHILUS-HARRISII; IMMUNOHISTOCHEMICAL CHARACTERIZATION; VENEREAL TUMOR; MICRORNAS; DIVERSITY; NEOPLASMS; EVOLUTION; PROTEIN; SYSTEM;
D O I
10.1126/science.1180616
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Tasmanian devil, a marsupial carnivore, is endangered because of the emergence of a transmissible cancer known as devil facial tumor disease (DFTD). This fatal cancer is clonally derived and is an allograft transmitted between devils by biting. We performed a large-scale genetic analysis of DFTD with microsatellite genotyping, a mitochondrial genome analysis, and deep sequencing of the DFTD transcriptome and microRNAs. These studies confirm that DFTD is a monophyletic clonally transmissible tumor and suggest that the disease is of Schwann cell origin. On the basis of these results, we have generated a diagnostic marker for DFTD and identify a suite of genes relevant to DFTD pathology and transmission. We provide a genomic data set for the Tasmanian devil that is applicable to cancer diagnosis, disease evolution, and conservation biology.
引用
收藏
页码:84 / 87
页数:4
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