RNA polymerase I propagates unidirectional spreading of rDNA silent chromatin

被引：74

作者：

Buck, SW ^{[1
]}

Sandmeier, JJ ^{[1
]}

Smith, JS ^{[1
]}

机构：

[1] Univ Virginia Hlth Syst, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA

来源：

CELL | 2002年 / 111卷 / 07期

关键词：

D O I：

10.1016/S0092-8674(02)01193-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ribosomal DNA (rDNA) tandem array in Saccharomyces cerevisiae induces transcriptional silencing of RNA polymerase II-transcribed genes. This SIR2-dependent form of repression (rDNA silencing) also functions to limit rDNA recombination and is involved in life span control. In this report, we demonstrate that rDNA silencing spreads into the centromere-proximal unique sequence located downstream of RNA polymerase I (Pol I) transcription, but fails to enter the upstream telomere-proximal sequences. The spreading of silencing correlates with SIR2-dependent histone H3 and H4 deacetylation and can be extended by SIR2 overexpression. Surprisingly, rDNA silencing required transcription by RNA polymerase I and the direction of spreading was controlled by the direction of Pol I transcription.

引用

页码：1003 / 1014

页数：12

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