Acetylation and accessibility of rDNA chromatin in Saccharomyces cerevisiae in Δtop1 and Δsir2 mutants

被引：14

作者：

Cioci, F

Vogelauer, M

Camilloni, G

机构：

[1] Univ Roma La Sapienza, Dipartimento Genet & Biol Mol, I-00185 Rome, Italy

[2] Univ Calif Los Angeles, Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Sch Med, Inst Mol Biol, Los Angeles, CA 90095 USA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2002年 / 322卷 / 01期

关键词：

chromatin; acetylation; DNA topoisomerase I; SIR2; rDNA;

D O I：

10.1016/S0022-2836(02)00749-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The insertion of reporter genes in the ribosomal DNA (rDNA) locus of Saccharomyces cerevisiae causes their transcriptional repression. This kind of transcriptional silencing depends on proteins such as Sir2p and Top1p, and has been shown to be mediated by chromatin. While Sir2p modifies nucleosomes directly through its histone deacetylase activity, little is known about changes in the chromatin structure that occur at the rDNA locus when TOP1 is deleted. Here, we show that the absence of Top1p causes increased histone acetylation at the rDNA locus. Moreover, rDNA chromatin becomes more accessible in a similar manner in both top1 and sir2 mutant strains. (C) 2002 Elsevier Science Ltd. All rights reserved.

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页码：41 / 52

页数：12

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