Expression of SHV-2 β-lactamase and of reduced amounts of OmpK36 porin in Klebsiella pneumoniae results in increased resistance to cephalosporins and carbapenems

被引：64

作者：

Crowley, B

Benedí, VJ

Doménech-Sánchez, A

机构：

[1] UIB, CSIC, IMEDEA, Microbiol Lab, Palma de Mallorca 07071, Spain

[2] Univ Hosp Aintree, Publ Hlth Lab, Liverpool L9 7AL, Merseyside, England

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2002年 / 46卷 / 11期

关键词：

D O I：

10.1128/AAC.46.11.3679-3682.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A Klebsiella pneumoniae clinical isolate was resistant to cefoxitin, cefotaxime, ceftazidime, ceftazidime-clavulanate, piperacillin-tazobactam (MICs, >256 mug/ml in all cases), and meropenem (MIC, 16 mug/ml) and was intermediate to imipenem (MIC, 8 mug/ml). Decreased expression of the OmpK36 porin and expression of an SHV-2 beta-lactamase contributed to the observed resistance to these beta-lactam-containing agents.

引用

收藏

页码：3679 / 3682

页数：4

相关论文

共 13 条

[1]

ALBERTI S, 1995, INFECT IMMUN, V63, P903

[2] Outer membrane profiles of clonally related Klebsiella pneumoniae isolates from clinical samples and activities of cephalosporins and carbapenems [J].

Ardanuy, C ;

Liñares, J ;

Domínguez, MA ;

Hernández-Allés, S ;

Benedi, VJ ;

Martínez-Martínez, L .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (07) :1636-1640

[3] Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC beta-lactamase, and the loss of an outer membrane protein [J].

Bradford, PA ;

Urban, C ;

Mariano, N ;

Projan, SJ ;

Rahal, JJ ;

Bush, K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (03) :563-569

[4]

Doménech-Sánchez A, 1999, J BACTERIOL, V181, P2726

[5] Crystal structure and functional characterization of OmpK36, the osmoporin of Klebsiella pneumoniae [J].

Dutzler, R ;

Rummel, G ;

Albertí, S ;

Hernández-Allés, S ;

Phale, PS ;

Rosenbusch, JP ;

Benedí, VJ ;

Schirmer, T .

STRUCTURE WITH FOLDING & DESIGN, 1999, 7 (04) :425-434

[6] Porin expression in clinical isolates of Klebsiella pneumoniae [J].

Hernández-Allés, S ;

Albertí, S ;

Alvarez, D ;

Doménech-Sánchez, A ;

Martínez-Martínez, L ;

Gil, J ;

Tomás, JM ;

Benedí, VJ .

MICROBIOLOGY-UK, 1999, 145 :673-679

[7] Acquired carbapenemases [J].

Livermore, DM .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1997, 39 (06) :673-676

[8] Emergence of a carbapenem-resistant Klebsiella pneumoniae [J].

MacKenzie, FM ;

Forbes, KJ ;

DoraiJohn, T ;

Amyes, SGB ;

Gould, IM .

LANCET, 1997, 350 (9080) :783-783

[9] Roles of β-lactamases and porins is activities of carbapenems and cephalosporins against Klebsiella pneumoniae [J].

Martínez-Martínez, L ;

Pascual, A ;

Hernández-Allés, S ;

Alvarez-Díaz, D ;

Suárez, AI ;

Tran, SJ ;

Benedi, VJ ;

Jacoby, GA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) :1669-1673

[10] In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins [J].

MartinezMartinez, L ;

HernandezAlles, S ;

Alberti, S ;

Tomas, JM ;

Benedi, VJ ;

Jacoby, GA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (02) :342-348