The human and mouse Period1 genes:: Five well-conserved E-boxes additively contribute to the enhancement of mPer1 transcription

被引：126

作者：

Hida, A

Koike, N

Hirose, M

Hattori, M

Sakaki, Y

Tei, H

机构：

[1] Univ Tokyo, Inst Med Sci, Ctr Human Genome, Lab Struct Genom,Minato Ku, Tokyo 1088639, Japan

[2] RIKEN, Genom Sci Ctr, Human Genome Res Grp, Sagamihara, Kanagawa 2288555, Japan

来源：

GENOMICS | 2000年 / 65卷 / 03期

关键词：

D O I：

10.1006/geno.2000.6166

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The clock gene, Period1, from human and mouse was sequenced and characterized. Both human PERIOD1 (human PER1) and mouse Period1 (mouse Per1) consisted of 23 exons spanning approximately 16 kb, and their structures showed strong similarity to each other. For example, six highly conserved regions were identified in the 5' upstream sequences. These conserved segments exhibited 77-88% identity and possessed several potential regulatory elements including five E-boxes (the binding site of the CLOCK-BMAL1 complex) and four cyclic AMP response elements. Transient transfection assays using a mPer1-luciferase fusion gene revealed that each of the conserved E-boxes additively functions as an enhancer for the transactivation of mPer1 by mCLOCK and mBMAL1. (C) 2000 Academic Press.

引用

页码：224 / 233

页数：10

共 32 条

[1] A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light [J].