The Onto- Tools suite is composed of an annotation database and eight complementary, web- accessible data mining tools: Onto- Express, Onto- Compare, Onto- Design, Onto- Translate, Onto- Miner, PathwayExpress, Promoter- Express and nsSNPCounter. Promoter- Express is a new tool added to the Onto- Toolsensemble that facilitates the identification of transcription factor binding sites active in specific conditions. nsSNPCounter is another new tool that allows computation and analysis of synonymous and non- synonymous codon substitutions for studying evolutionary rates of protein coding genes. Onto- Translate has also been enhanced to expand its scope and accuracy by fully utilizing the capabilities of the Onto- Tools database. Currently, Onto- Translate allows arbitrary mappings between 28 types of IDs for 53 organisms. Onto- Tools are freely available at http://vortex.cs.wayne.edu/Projects.html.