The Biocompatibility of Silver-Containing Na2O•CaO•2SiO2 Glass Prepared by Sol-Gel Method: In Vitro Studies

被引：25

作者：

Raucci, Maria Grazia ^{[1
]}

Adesanya, Kehinde ^{[2
]}

Di Silvio, Lucy ^{[2
]}

Catauro, Michelina ^{[3
]}

Ambrosio, Luigi ^{[1
]}

机构：

[1] CNR, Inst Composite & Biomed Mat, IMCB, I-80125 Naples, Italy

[2] Kings Coll London, Inst Dent, Dept Biomat, Biomimet & Biophoton Grp, London SE1 9KT, England

[3] Univ Naples 2, Dept Mech & Aerospatiale Engn, I-81031 Aversa, Italy

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | 2010年 / 92B卷 / 01期

关键词：

silver-containing gel glass; antibacterial material; sterilization methods; MTT assay; alamar blue; DRUG-DELIVERY SYSTEM; ALAMAR BLUE; ANTIBACTERIAL; MTT; INFECTION; CYTOTOXICITY; BIOACTIVITY; BIOFILMS;

D O I：

10.1002/jbm.b.31495

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

This study was performed to determine the in vitro biocompatibility of a bioactive, antibacterial silver based gel. A human osteoblast-like cell line (HOS TE85) was used to test cytocompatibility in the presence of eluants and the biological response in direct contact with Na2O center dot CaO center dot 2SiO(2) gel (containing 0.5050 wt % Ag2O prepared by a sol-gel process) sterilized by two different methods; autoclave and gamma-irradiation. MTT assay was used for the indirect evaluation of eluant toxicity and alamar Blue (TM) was performed to assess the biological response, which included cell viability and proliferation, and the results showed that these materials were cytocompatible. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part 13: Appl Biomater 92B: 102-110, 2010

引用

页码：102 / 110

页数：9

共 36 条

[1]

Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT [J].

Abe, K ;

Matsuki, N .

NEUROSCIENCE RESEARCH, 2000, 38 (04) :325-329

[2]

The Erlanger silver catheter:: In vitro results for antimicrobial activity [J].

Bechert, T ;

Böswald, M ;

Lugauer, S ;

Regenfus, A ;

Greil, J ;

Guggenbichler, JP .

INFECTION, 1999, 27 (Suppl 1) :S24-S29

[3]

DYNAMICS OF CELL-MEMBRANE PERMEABILITY CHANGES AT SUPRAPHYSIOLOGICAL TEMPERATURES [J].

BISCHOF, JC ;

PADANILAM, J ;

HOLMES, WH ;

EZZELL, RM ;

LEE, RC ;

TOMPKINS, RG ;

YARMUSH, ML ;

TONER, M .

BIOPHYSICAL JOURNAL, 1995, 68 (06) :2608-2614

[4]

Silver coated materials for external fixation devices:: in vitro biocompatibility and genotoxicity [J].

Bosetti, M ;

Massè, A ;

Tobin, E ;

Cannas, M .

BIOMATERIALS, 2002, 23 (03) :887-892

[5]

Novel periodontal drug delivery system for treatment of periodontitis [J].

Bromberg, LE ;

Buxton, DK ;

Friden, PM .

JOURNAL OF CONTROLLED RELEASE, 2001, 71 (03) :251-259

[6]

Release kinetics of ampicillin, characterization and bioactivity of TiO2/PCL hybrid materials synthesized by sol-gel processing [J].

Catauro, M ;

Raucci, MG ;

De Marco, D ;

Ambrosio, L .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2006, 77A (02) :340-350

[7]

Sol-gel synthesis, structure and bioactivity of polycaprolactone/CaO•SiO2 hybrid material [J].

Catauro, M ;

Raucci, MG ;

De Gaetano, F ;

Buri, A ;

Marotta, A ;

Ambrosio, L .

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2004, 15 (09) :991-995

[8]

Antibacterial and bioactive silver-containing Na2O • CaO • 2SiO2 glass prepared by sol-gel method [J].

Catauro, M ;

Raucci, MG ;

De Gaetano, F ;

Marotta, A .

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2004, 15 (07) :831-837

[9]

Biocompatibility tests with fibroblasts of CaO rich calcium silicate glasses [J].

Catauro, M ;

Raucci, MG ;

Continenza, MA ;

Marotta, A .

JOURNAL OF MATERIALS SCIENCE, 2004, 39 (01) :373-375

[10]

The development and functions of silver in water purification and disease control [J].

Davies, RL ;

Etris, SF .

CATALYSIS TODAY, 1997, 36 (01) :107-114

← 1 2 3 4 →